Anti-MHC Class II antibody (ab55152)
Key features and details
- Mouse monoclonal to MHC Class II
- Suitable for: IHC-P, ICC/IF, WB, Flow Cyt
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2a
Overview
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Product name
Anti-MHC Class II antibody
See all MHC Class II primary antibodies -
Description
Mouse monoclonal to MHC Class II -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB HumanRecombinant fragment
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Immunogen
Recombinant full length protein corresponding to Human MHC Class II aa 1-258.
Database link: P04440 -
Positive control
- This antibody gave a positive result when used in the following formaldehyde fixed cell lines: A431
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General notes
This product was changed from ascites to tissue culture supernatant on 13th Feb 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4 -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MHC Class II antibody (ab55152) This image is courtesy of an anonymous Abreview
ab55152 staining MHC Class II in Human bowel tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 1 hour at 21°C; antigen retrieval was enzymatic. Samples were incubated with primary antibody (1/50 in milk) for 21 hours at 4°C. A Biotin-conjugated Rabbit anti-mouse polyclonal (1/300) was used as the secondary antibody.
This image was generated using the ascites version of the product.
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Immunocytochemistry/ Immunofluorescence - Anti-MHC Class II antibody (ab55152)ICC/IF image of ab55152 stained A431 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab55152 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This image was generated using the ascites version of the product.
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Western blot - Anti-MHC Class II antibody (ab55152)All lanes : Anti-MHC Class II antibody (ab55152)
Lane 1 : MHC Class II transfected 293T cell lysates
Lane 2 : Non-transfected 293T cell lysates
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Western blot - Anti-MHC Class II antibody (ab55152)Western blot against tagged recombinant protein immunogen using ab55152 MHC Class II antibody at 1ug/ml. Predicted band size of immunogen is 54 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MHC Class II antibody (ab55152)MHC Class II antibody (ab55152) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human lymph node.
This image was generated using the ascites version of the product.
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Flow Cytometry - Anti-MHC Class II antibody (ab55152)Overlay histogram showing Raji cells stained with ab55152 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55152, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Raji cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
This image was generated using the ascites version of the product.
