Anti-MHC Class II antibody (ab180779)
Key features and details
- Rabbit polyclonal to MHC Class II
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-MHC Class II antibody
See all MHC Class II primary antibodies -
Description
Rabbit polyclonal to MHC Class II -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human MHC Class II aa 30-225. Extracellular domain.
Sequence:RATPENYLFQGRQECYAFNGTQRFLERYIYNREEFARFDSDVGEFRAVTE LGRPAAEYWN SQKDILEEKRAVPDRMCRHNYELGGPMTLQRRVQPRVN VSPSKKGPLQHHNLLVCHVTDF YPGSIQVRWFLNGQEETAGVVSTNLI RNGDWTFQILVMLEMTPQQGDVYTCQVEHTSLDS PVTVEWKAQSDSAR SK
Database link: P04440 -
Positive control
- WB: U-937 and THP-1 cell lysates IHC: Human lymph node and stomach tissues.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MHC Class II antibody (ab180779)
Paraffin-embedded human stomach tissue stained for MHC Class II using ab180779 at 1/100 dilution in immunohistochemical analysis.
-
Western blot - Anti-MHC Class II antibody (ab180779)All lanes : Anti-MHC Class II antibody (ab180779) at 1/1000 dilution
Lane 1 : U-937 cell lysate
Lane 2 : THP-1 cell lysate
Lane 3 : Mouse spleen tissue lysate
Lane 4 : Mouse thymus tissue lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L)
Predicted band size: 29 kDaBlocking buffer: 3% nonfat dry milk in TBST.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MHC Class II antibody (ab180779)Paraffin-embedded human lymph node tissue stained for MHC Class II using ab180779 at 1/100 dilution in immunohistochemical analysis.
