Anti-OMA1 antibody (ab154949)
Key features and details
- Rabbit polyclonal to OMA1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-OMA1 antibody -
Description
Rabbit polyclonal to OMA1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Sheep, Rabbit, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan -
Immunogen
Synthetic peptide within Human OMA1 aa 400-500 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q96E52 -
Positive control
- This antibody gave a positive signal in Human, Mouse and Rat Heart tissue lysates. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal kidney.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentrationConcentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154949 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB (2) Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 60 kDa).IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 60 kDa).IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.Target
-
Function
Mitochondrial protease. -
Tissue specificity
Widely expressed, with strong expression in the heart, skeletal muscle, kidney and liver. -
Sequence similarities
Belongs to the peptidase M48 family. -
Cellular localization
Mitochondrion membrane. - Information by UniProt
-
Database links
- Entrez Gene: 115209 Human
- Entrez Gene: 67013 Mouse
- Entrez Gene: 298282 Rat
- SwissProt: Q96E52 Human
- SwissProt: Q9D8H7 Mouse
- SwissProt: D3ZS74 Rat
- Unigene: 425769 Human
- Unigene: 30021 Mouse
-
Alternative names
- 2010001O09Rik antibody
- DAB1 antibody
- FLJ33782 antibody
see all
Images
-
IHC image of OMA1 staining in human normal kidney formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab154949, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
-
All lanes : Anti-OMA1 antibody (ab154949) at 1 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431)
Lane 2 : Heart (Mouse) Tissue Lysate
Lane 3 : Heart (Rat) Tissue Lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?
Additional bands at: 82 kDa (possible non-specific binding)
Exposure time: 90 secondsThe band observed at 58 kDa could potentially be a cleaved form of OMA1 due to the presence of a 13 amino acid transit peptide.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab154949 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (3)
ab154949 has been referenced in 3 publications.
- Li X et al. Ischemia-induced cleavage of OPA1 at S1 site aggravates mitochondrial fragmentation and reperfusion injury in neurons. Cell Death Dis 13:321 (2022). PubMed: 35395832
- Pang Y et al. HIGD-1B inhibits hypoxia-induced mitochondrial fragmentation by regulating OPA1 cleavage in cardiomyocytes. Mol Med Rep 24:N/A (2021). PubMed: 34080026
- Axelrod CL et al. Exercise training remodels human skeletal muscle mitochondrial fission and fusion machinery towards a pro-elongation phenotype. Acta Physiol (Oxf) N/A:e13216 (2018). PubMed: 30408342
Images
-
IHC image of OMA1 staining in human normal kidney formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab154949, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
-
All lanes : Anti-OMA1 antibody (ab154949) at 1 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431)
Lane 2 : Heart (Mouse) Tissue Lysate
Lane 3 : Heart (Rat) Tissue Lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?
Additional bands at: 82 kDa (possible non-specific binding)
Exposure time: 90 secondsThe band observed at 58 kDa could potentially be a cleaved form of OMA1 due to the presence of a 13 amino acid transit peptide.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab154949 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406