Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PPCEL/PREPL antibody [EPR17751] (ab202064)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17751] to PPCEL/PREPL
  • Suitable for: WB, ICC/IF, IP, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PPCEL/PREPL antibody [EPR17751]
    See all PPCEL/PREPL primary antibodies

  • Description

    Rabbit monoclonal [EPR17751] to PPCEL/PREPL

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC/IF
    Human
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: MCF-7, NIH/3T3, A673 cell lysate, L6 cell lysate, Mouse brain lysate, Human fetal brain lysate, Human fetal heart lysate, Human fetal kidney lysate, C6 cell lysate, Raw264.7 cell lysate, PC12 cell lysate, Human PPCEL/PREPL full length protein; ICC/IF: SH-SY5Y and MCF-7 cells; Flow Cyt: NIH/3T3 cells; IP: MCF-7 cell lysate.

  • General notes

     

     

     This product was previously labelled as PPCEL

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR17751

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-PPCEL/PREPL antibody [EPR17751] (ab202064)
    Western blot - Anti-PPCEL/PREPL antibody [EPR17751] (ab202064)
    Anti-PPCEL/PREPL antibody [EPR17751] (ab202064) at 1/10000 dilution + Recombinant Human PPCEL/PREPL protein (ab160557)

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 84 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 seconds


    Blocking and diluting buffer was 5% NFDM/TBS

  • Immunoprecipitation - Anti-PPCEL/PREPL antibody [EPR17751] (ab202064)
    Immunoprecipitation - Anti-PPCEL/PREPL antibody [EPR17751] (ab202064)

    PPCEL/PREPL was immunoprecipitated from 1mg of MCF7 (Human breast adenocarcinoma cell line) whole cell extract with ab202064 at 1/40.

    Western blot was performed using ab202064 at 1/2000.

    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: MCF7 whole cell lysate at 10ug.

    Lane 2: Immunoprecipitate of MCF7 whole cell lysate using ab202064.

    Lane 3: Immunoprecipitate of MCF7 whole cell lysate using ab172730 (Rabbit monoclonal IgG).
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  • Flow Cytometry - Anti-PPCEL/PREPL antibody [EPR17751] (ab202064)
    Flow Cytometry - Anti-PPCEL/PREPL antibody [EPR17751] (ab202064)

    Flow cytometric analysis of 4% paraformaldehyde-fixed NIH/3T3 (Mouse embryo fibroblast) cells labeling PPCEL/PREPL with ab202064 at 1/150 (red) compared with a rabbit monoclonal IgG isotype control (ab172730) (black) and a unlabelled control (cells without incubation with primary antibody and secondary antibody (blue)). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-PPCEL/PREPL antibody [EPR17751] (ab202064)
    Immunocytochemistry/ Immunofluorescence - Anti-PPCEL/PREPL antibody [EPR17751] (ab202064)

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized SH-SY5Y (Human neuroblastoma from bone marrow cells) cells labeling PPCEL/PREPL with ab202064 at 1/1000, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 (green). Confocal image showing cytoplasmic staining on SH-SY5Y cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) at 1/500 (red).
    The negative controls are as follows:-
    -ve control 1 - ab202064 at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/500 dilution.
    -ve control 2. - Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-PPCEL/PREPL antibody [EPR17751] (ab202064)
    Immunocytochemistry/ Immunofluorescence - Anti-PPCEL/PREPL antibody [EPR17751] (ab202064)

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling PPCEL/PREPL with ab202064 at 1/1000, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 (green). Confocal image showing cytoplasmic staining on MCF-7 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/500 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab202064 at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary at 1/500 dilution.
    -ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary at 1/500 dilution.

  • Western blot - Anti-PPCEL/PREPL antibody [EPR17751] (ab202064)
    Western blot - Anti-PPCEL/PREPL antibody [EPR17751] (ab202064)
    All lanes : Anti-PPCEL/PREPL antibody [EPR17751] (ab202064) at 1/1000 dilution

    Lane 1 : C6 (Rat glial tumor cells) lysate
    Lane 2 : Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus)) lysate
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) lysate
    Lane 4 : NIH/3T3 (Mouse embryo fibroblast cells) lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 84 kDa
    Observed band size: 72 kDa why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Blocking and diluting buffer was 5% NFDM/TBS

  • Western blot - Anti-PPCEL/PREPL antibody [EPR17751] (ab202064)
    Western blot - Anti-PPCEL/PREPL antibody [EPR17751] (ab202064)
    All lanes : Anti-PPCEL/PREPL antibody [EPR17751] (ab202064) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 84 kDa
    Observed band size: 72 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking and diluting buffer was 5% NFDM/TBS

  • Western blot - Anti-PPCEL/PREPL antibody [EPR17751] (ab202064)
    Western blot - Anti-PPCEL/PREPL antibody [EPR17751] (ab202064)
    Anti-PPCEL/PREPL antibody [EPR17751] (ab202064) at 1/5000 dilution + Mouse brain lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 84 kDa
    Observed band size: 72 kDa why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Blocking and diluting buffer was 5% NFDM/TBS

  • Western blot - Anti-PPCEL/PREPL antibody [EPR17751] (ab202064)
    Western blot - Anti-PPCEL/PREPL antibody [EPR17751] (ab202064)
    All lanes : Anti-PPCEL/PREPL antibody [EPR17751] (ab202064) at 1/5000 dilution

    Lane 1 : A673 (Human muscle Ewing's Sarcoma cell line) lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line ) lysate
    Lane 3 : L6 (Rat skeletal muscle cell line) lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 84 kDa
    Observed band size: 72 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking and diluting buffer was 5% NFDM/TBST

  • Anti-PPCEL/PREPL antibody [EPR17751] (ab202064)
    Anti-PPCEL/PREPL antibody [EPR17751] (ab202064)

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