Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: ATRX knockout HAP1 whole cell lysate (20 µg)

Lanes 1 - 2: Merged signal (red and green). Green - ab188027 observed at 300 kDa. Red - loading control, ab176560, observed at 50 kDa.

ab188027 was shown to recognize ATRX in wild type cells as signal was lost at the expected MW in ATRX knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ATRX knockout samples were subjected to SDS-PAGE. Ab188027 and ab176560 (Rabbit anti alpha Tubulin loading control) were incubated overnight at 4°C at a 1/500 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

 

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human glioma tissue labelling ATRX with ab188027 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/Immunofluorescence analysis of A431 cells labelling ATRX with ab188027 showing clear nuclear (without nucleoli) staining in green. Microtubule are visualized in red.

All lanes : Anti-ATRX antibody [CL0537] (ab188027)

Lane 1 : A-549 cells transfected with control siRNA
Lane 2 : A-549 cells transfected with target specific siRNA probe #1
Lane 3 : A-549 cells transfected with target specific siRNA probe #2

Predicted band size: 282 kDa

Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fallopian tube tissue labelling ATRX with ab188027 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling ATRX with ab188027 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling ATRX with ab188027 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling ATRX with ab188027 showing clear nuclear (without nucleoli) staining in green. Microtubule are visualized in red.