Anti-ATRX antibody (ab204169)
Key features and details
- Rabbit polyclonal to ATRX
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ATRX antibody
See all ATRX primary antibodies -
Description
Rabbit polyclonal to ATRX -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chimpanzee
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Immunogen
Recombinant fragment corresponding to Human ATRX aa 2250-2450.
Database link: P46100 -
Positive control
- WB: A549 whole cell lysates; IHC-P: Human uterine cervix, glioma, cerebral cortex tissue; ICC :A549 whole cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ATRX antibody (ab204169)All lanes : Anti-ATRX antibody (ab204169) at 0.4 µg/ml
Lane 1 : Control siRNA - A549 (Human lung carcinoma cell line) whole cell lysate
Lane 2 : siRNA probe #1 - A549 whole cell lysate
Lane 3 : siRNA probe #2 - A549 whole cell lysate
Predicted band size: 282 kDaLoading control: Anti-GAPDH.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATRX antibody (ab204169)Immunohistochemical analysis of human uterine cervix tissue labeling ATRX in the nucleus of squamous epithelial cells with ab204169 at a 1/500 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry/ Immunofluorescence - Anti-ATRX antibody (ab204169)Immunocytochemical analysis of A549 (Human lung carcinoma cell line) whole cells labeling ATRX in the nucleoplasm and nuclear bodies with ab204169 at 2 µg/ml.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATRX antibody (ab204169)Immunohistochemical analysis of human glioma tissue labeling ATRX in the nucleus of tumor cells with ab204169 at a 1/500 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATRX antibody (ab204169)Immunohistochemical analysis of human cerebral cortex tissue labeling ATRX in the nucleus of neurons and glial cells with ab204169 at a 1/500 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATRX antibody (ab204169)Immunohistochemical analysis of human liver tissue labeling ATRX in the hepatocytes with ab204169 at a 1/500 dilution. No positivity as expected.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
