Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-KMT3A / HYPB / HIF-1 antibody [EPR20927-67] - BSA and Azide free (ab274329)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20927-67] to KMT3A / HYPB / HIF-1 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-KMT3A / HYPB / HIF-1 antibody [EPR20927-67] - BSA and Azide free
    See all KMT3A / HYPB / HIF-1 primary antibodies

  • Description

    Rabbit monoclonal [EPR20927-67] to KMT3A / HYPB / HIF-1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: MEF and HepG2 whole cell lysates. HeLa nuclear fraction.

  • General notes

    ab274329 is the carrier-free version of ab239350. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-KMT3A / HYPB / HIF-1 antibody [EPR20927-67] - BSA and Azide free (ab274329)
    Western blot - Anti-KMT3A / HYPB / HIF-1 antibody [EPR20927-67] - BSA and Azide free (ab274329)
    All lanes : Anti-KMT3A / HYPB / HIF-1 antibody [EPR20927-67] (ab239350) at 1/1000 dilution

    Lane 1 : MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate
    Lane 2 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 288 kDa
    Observed band size: 288 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure times: Lane 1: 26 seconds; Lane 2: 86 seconds.  

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, glycerol, BSA and sodium azide (ab239350).

  • Western blot - Anti-KMT3A / HYPB / HIF-1 antibody [EPR20927-67] - BSA and Azide free (ab274329)
    Western blot - Anti-KMT3A / HYPB / HIF-1 antibody [EPR20927-67] - BSA and Azide free (ab274329)
    Anti-KMT3A / HYPB / HIF-1 antibody [EPR20927-67] (ab239350) at 1/1000 dilution + HeLa (human cervix adenocarcinoma epithelial cell) nuclear fraction at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 288 kDa
    Observed band size: 288 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, glycerol, BSA and sodium azide (ab239350).

  • Anti-KMT3A / HYPB / HIF-1 antibody [EPR20927-67] - BSA and Azide free (ab274329)
    Anti-KMT3A / HYPB / HIF-1 antibody [EPR20927-67] - BSA and Azide free (ab274329)

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