Recombinant human KMT1A / SUV39H1 protein (ab80289)
Key features and details
- Expression system: Escherichia coli
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human KMT1A / SUV39H1 protein
See all KMT1A / SUV39H1 proteins and peptides -
Biological activity
Specific Activity: 3.5 pmol/min/μg.
Assay conditions: 50 μl reaction mix (50 mM TRIS pH 8.8, 5mM MgCl2, 4mM DTT, 0.05% Tween-20, 40 μM S-adenosylmethionine, and 10-100 ng SUV39H1) add to the wells coated with the substrate on a GBioscience Neutravidin black plate. Incubate for 1 hr. Add antibody against methylated K9 residue of histone H3, incubate 1 hr. Then, add secondary HRP-labeled antibody and
incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminescence. -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
64 kDa including tags -
Amino acids
82 to 412 -
Tags
GST tag N-Terminus
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Preparation and Storage
- H3 K9 HMTase1
- H3-K9-HMTase 1
- Histone H3-K9 methyltransferase 1
Images
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Functional Studies - Recombinant human KMT1A / SUV39H1 protein (ab80289)
Image showing specific activity of ab80289.
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SDS-PAGE - Recombinant human KMT1A / SUV39H1 protein (ab80289)10% SDS-PAGE showing ab80289 at approximately 64kDa (3µg). 2μg of protein loaded.