Alpha-L-Fucosidase Activity Assay Kit (Fluorometric) (ab273324)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Fluorescent
- Platform: Microplate
- Sample type: Plasma, Serum
- Sensitivity: 1 µU
Overview
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Product name
Alpha-L-Fucosidase Activity Assay Kit (Fluorometric)
See all FUCA1 kits -
Detection method
Fluorescent -
Sample type
Serum, Plasma -
Assay type
Enzyme activity (quantitative) -
Sensitivity
1 µU -
Assay duration
Multiple steps standard assay -
Product overview
Alpha-L-Fucosidase Activity Assay Kit (Fluorometric) (ab273324) provides a simple, sensitive and high-throughput adaptable approach to detect physiological concentrations of this glycosidase in a variety of biological samples. In this assay, Alpha-L-Fucosidase (FUCA1) uses a synthetic 4-MUF substrate and releases a fluorescent methylumbelliferyl derivative (4- MU) that can be measured kinetically under acidic conditions (Ex/Em = 330/450 nm). The assay is a single-step reaction, with minimal sample preparation.
The assay can detect less than 1 µU/ml of FUCA1 activity in serum samples.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Tested applications
Suitable for: Othermore details -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests 4-MU Standard (5 mM in DMSO) 1 x 35µl 4-MUF Substrate (in DMSO) 1 x 55µl DTT (1 M) 1 x 1ml FUCA1 Assay Buffer 1 x 25ml FUCA1 Positive Control 1 x 20µl -
Research areas
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Function
Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins. -
Involvement in disease
Defects in FUCA1 are the cause of fucosidosis (FUCA1D) [MIM:230000]. FUCA1D is an autosomal recessive lysosomal storage disease characterized by accumulation of fucose-containing glycolipids and glycoproteins in various tissues. Clinical signs include facial dysmorphism, dysostosis multiplex, moderate hepatomegaly, severe intellectual deficit, deafness, and according to age, angiokeratomas. -
Sequence similarities
Belongs to the glycosyl hydrolase 29 family. -
Cellular localization
Lysosome. - Information by UniProt
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Alternative names
- Alpha L fucosidase I
- Alpha L fucoside fucohydrolase
- Alpha-L-fucosidase 1
see all
Images
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4-MU Standard Curve
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
4-MU Standard Curve.
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Measurement of activity in FUCA1Example data.
Measurement of activity in FUCA1, serum from normal patient and serum from breast cancer patient; and background contributed by assay buffer.
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Estimation of FUCA1 in human serumExample data.
Estimation of FUCA1 in human serum from normal patient and patient with breast cancer. Five microliters of each undiluted sample were assayed following kit protocol.