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Signal Transduction Metabolism Amino Acids

Anti-CARS antibody [EPR7121] - BSA and Azide free (ab248142)

Price and availability

526 012 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Anti-CARS antibody [EPR7121] - BSA and Azide free (ab248142)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
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  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7121] to CARS - BSA and Azide free
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-CARS antibody [EPR7121] - BSA and Azide free
    See all CARS primary antibodies
  • Description

    Rabbit monoclonal [EPR7121] to CARS - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248142 is the carrier-free version of ab126714 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab248142 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Dissociation constant (KD)

    KD = 5.70 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR7121
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • tRNA synthetase
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Nucleotide metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism

Images

  • Western blot - Anti-CARS antibody [EPR7121] - BSA and Azide free (ab248142)
    Western blot - Anti-CARS antibody [EPR7121] - BSA and Azide free (ab248142)
    All lanes : Anti-CARS antibody [EPR7121] (ab126714) at 1/10000 dilution

    Lane 1 : 293T (Human embryonic kidney epithelial cell) cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate
    Lane 4 : BxPC-3 cell lysate
    Lane 5 : MCF-7 cell lysate
    Lane 6 : A549 cell lysate

    Predicted band size: 85 kDa



    This data was developed using ab126714, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARS antibody [EPR7121] - BSA and Azide free (ab248142)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARS antibody [EPR7121] - BSA and Azide free (ab248142)
    This data was developed using ab126714, the same antibody clone in a different buffer formulation.ab126714, at 1/50 dilution, stains CARS in paraffin-embedded human breast carcinoma tissue by immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • OI-RD Scanning - Anti-CARS antibody [EPR7121] - BSA and Azide free (ab248142)
    OI-RD Scanning - Anti-CARS antibody [EPR7121] - BSA and Azide free (ab248142)
    This data was developed using ab126714, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-CARS antibody [EPR7121] - BSA and Azide free (ab248142)
    Anti-CARS antibody [EPR7121] - BSA and Azide free (ab248142)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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