Anti-CACNA1A antibody [EPR4458(N)] - BSA and Azide free (ab240251)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4458(N)] to CACNA1A - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-CACNA1A antibody [EPR4458(N)] - BSA and Azide free
See all CACNA1A primary antibodies -
Description
Rabbit monoclonal [EPR4458(N)] to CACNA1A - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab205923) -
General notes
Ab240251 is the carrier-free version of ab181371. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab240251 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4458(N) -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-CACNA1A antibody [EPR4458(N)] (ab181371) at 1/5000 dilution
Lane 1 : Human cerebellum tissue lysate
Lane 2 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysate
Lane 3 : Human fetal brain tissue lysate
Lane 4 : C6 (Rat glial tumor glial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 282 kDaThis data was developed using ab181371, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
The 220 and 170 kDa is full-length a1A subunit species. The 60 kDa is a C-terminal fragment of the a1A subunit (PMID: 16595610).
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All lanes : Anti-CACNA1A antibody [EPR4458(N)] (ab181371) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 282 kDaThis data was developed using ab181371, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
The 220 and 170 kDa is full-length a1A subunit species. The 60 kDa is a C-terminal fragment of the a1A subunit (PMID: 16595610).
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All lanes : Anti-CACNA1A antibody [EPR4458(N)] (ab181371) at 1/1000 dilution
Lane 1 : Human fetal brain lysates
Lane 2 : Human cerebellum lysates
Lane 3 : C6 lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 282 kDaThis data was developed using ab181371, the same antibody clone in a different buffer formulation.
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