Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human MRP1 ELISA Kit (ABCC1) (ab272211)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 0.151 ng/ml
  • Range: 0.63 ng/ml - 40 ng/ml
  • Sample type: Cell Lysate, Tissue Extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human MRP1 ELISA Kit (ABCC1)
    See all MRP1 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Extract 8 2.9%

  • Sample type

    Tissue Extracts, Cell Lysate

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    0.151 ng/ml

  • Range

    0.63 ng/ml - 40 ng/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Tissue Extracts 95 94% - 96%
    Cell Lysate 94 91% - 97%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human MRP1 ELISA kit (ab272211) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MRP1 protein in cell and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human MRP1 with 151 pg/mL sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            -Single-wash protocol reduces assay time to 90 minutes or less


            -High sensitivity, specificity and reproducibility from superior antibodies


            -Fully validated in biological samples


            -96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    MRP1 (Multidrug Resistance-associated Protein 1), also known as ABCC1 (ATP-Binding Cassette sub-family C member 1), mediates export of organic anions and drugs from the cytoplasm. It mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. MRP1 confers resistance to anticancer drugs by decreasing accumulation of drug in cells, and by mediating ATP- and GSH-dependent drug export.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • SimpleStep ELISA Protocol Diagram
    SimpleStep ELISA Protocol Diagram

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

  • Example of human MRP1 standard curve in 1X Cell Extractio Buffer PTR.
    Example of human MRP1 standard curve in 1X Cell Extractio Buffer PTR.

    The MRP1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native MRP1 in human A431 cell, HeLa cell and lung tissue based on 150 µg/mL, 600 µg/mL and 1,000 µg/mL extract loads, respectively.
    Interpolated concentrations of native MRP1 in human A431 cell, HeLa cell and lung tissue based on 150 µg/mL, 600 µg/mL and 1,000 µg/mL extract loads, respectively.

    The concentrations of MRP1 were measured in duplicate and interpolated from the MRP1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MRP1 concentration was determined to be 13.6 ng/mL in A431 extract, 24.1 ng/mL in HeLa extract and 5.1 ng/mL in lung extract.

  • Interpolated concentrations of native MRP1 in human control wild type HeLa cell and MRP1 knockout HeLa cell based on 200 µg/mL extract loads.
    Interpolated concentrations of native MRP1 in human control wild type HeLa cell and MRP1 knockout HeLa cell based on 200 µg/mL extract loads.

    The concentrations of MRP1 were measured in duplicate and interpolated from the MRP1 standard curve and expressing the interpolated concentrations as ng of protein per mg of extract (mean +/- SD, n=2). The mean MRP1 concentration was determined to be 11.8 ng/mg in wild type HeLa extract and undetectable in MRP1 knockout HeLa extract.

  • Sandwich ELISA - Human MRP1 ELISA Kit (ABCC1) (ab272211)
    Sandwich ELISA - Human MRP1 ELISA Kit (ABCC1) (ab272211)
    To learn more about the advantages of recombinant antibodies see here.

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