Anti-REEP2 antibody [S326D-2] (ab242000)
![Anti-REEP2 antibody [S326D-2] (ab242000)](https://www.abcam.com/ps/products/242/ab242000/Images/ab242000-331827-000a.jpg "Anti-REEP2 antibody [S326D-2] (ab242000)")
Key features and details
- Mouse monoclonal [S326D-2] to REEP2
- Suitable for: WB, ICC/IF
- Reacts with: Human, African green monkey
- Isotype: IgG2a
Overview
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Product name
Anti-REEP2 antibody [S326D-2]
See all REEP2 primary antibodies -
Description
Mouse monoclonal [S326D-2] to REEP2 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human, African green monkey -
Immunogen
Fusion protein corresponding to Mouse REEP2 aa 100 to the C-terminus. (Cytoplasmic C-terminus)
Database link: Q8VCD6 -
Positive control
- WB: COS over expressing REEP2-His. ICC/IF: SK-N-BE cells.
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General notes
This product was previously labelled as C5orf19
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
S326D-2 -
Isotype
IgG2a -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-REEP2 antibody [S326D-2] (ab242000)
4% formaldehyde-fixed SK-N-BE (human neuroblastoma cell line) cells stained for REEP2 (green, C) using ab242000 at 1/100 dilution for 1 hour at room temperature in ICC/IF. Secondary antibody is Goat Anti-Mouse ATTO 488 at 1/100 dilution at a 1 hr at RT. Actin is detected with Phalloidin Texas Red F-Actin stain (red, B). The nuclear counterstain is DAPI (blue, A). (D) is a composite image.
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![Western blot - Anti-REEP2 antibody [S326D-2] (ab242000)](https://www.abcam.com/ps/products/242/ab242000/Images/ab242000-331828-000b.jpg)
Western blot - Anti-REEP2 antibody [S326D-2] (ab242000)All lanes : Anti-REEP2 antibody [S326D-2] (ab242000) at 1/1000 dilution
Lane 1 : COS overexpressing REEP1-His cell lysate at 15 µg
Lane 2 : COS overexpressing REEP2-His cell lysate
Lane 3 : COS overexpressing REEP6-His cell lysate
Secondary
All lanes : Goat Anti-Mouse IgG: HRP at 1/200 dilution
Developed using the ECL technique.
Predicted band size: 28 kDa
Exposure time: 6 minutesPrimary incubation for 16 hours at 4°C.
Secondary incubation for 1 hr at RT.
Blocked for 1 hr at RT.
Note: C5orf19 = REEP2
