Anti-Muscarinic Acetylcholine Receptor M4/CHRM4 antibody [18C7.2] (ab77956)
![Anti-Muscarinic Acetylcholine Receptor M4/CHRM4 antibody [18C7.2] (ab77956)](https://www.abcam.com/ps/products/77/ab77956/Images/Muscarinic-Acetylcholine-Receptor-M4-Primary-antibodies-ab77956-6.jpg "Anti-Muscarinic Acetylcholine Receptor M4/CHRM4 antibody [18C7.2] (ab77956)")
Key features and details
- Mouse monoclonal [18C7.2] to Muscarinic Acetylcholine Receptor M4/CHRM4
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Muscarinic Acetylcholine Receptor M4/CHRM4 antibody [18C7.2]
See all Muscarinic Acetylcholine Receptor M4/CHRM4 primary antibodies -
Description
Mouse monoclonal [18C7.2] to Muscarinic Acetylcholine Receptor M4/CHRM4 -
Host species
Mouse -
Specificity
ab77956 reacts with Muscarinic Acetylcholine Receptor M4/CHRM4. There is no reactivity with the other subtypes.
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Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Muscarinic Acetylcholine Receptor M4/CHRM4.
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Positive control
- Transfected CHO cell lines
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General notes
This product was previously labelled as Muscarinic Acetylcholine Receptor M4
This product was changed from ascites to tissue culture supernatant on 21 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Properties
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Form
Liquid -
Storage instructions
Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 1.45% Sodium chloride, 0.0536% PBS -
Concentration information loading... -
Purity
Tissue culture supernatant -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
18C7.2 -
Isotype
IgG1 -
Research areas
Images
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Flow Cytometry - Anti-Muscarinic Acetylcholine Receptor M4/CHRM4 antibody [18C7.2] (ab77956)
Overlay histogram showing SH-SY5Y cells stained with ab77956 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77956, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This image was generated using the ascites version of the product.
