HRP Anti-Histone H3 antibody - Nuclear Loading Control (ab21054)
Key features and details
- HRP Rabbit polyclonal to Histone H3 - Nuclear Loading Control
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human, Saccharomyces cerevisiae, Drosophila melanogaster
- Conjugation: HRP
- Isotype: IgG
Overview
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Product name
HRP Anti-Histone H3 antibody - Nuclear Loading Control
See all Histone H3 primary antibodies -
Description
HRP Rabbit polyclonal to Histone H3 - Nuclear Loading Control -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human, Saccharomyces cerevisiae, Drosophila melanogaster -
Immunogen
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Positive control
- This antibody gave a positive signal in the following: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Yeast S.cerevisiae Whole Cell Lysate Drosophila Embryo Nuclear Extract IHC-P: FFPE human skin (normal) and human cervix (normal) tissue sections.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - HRP Anti-Histone H3 antibody - Nuclear Loading Control (ab21054)All lanes : HRP Anti-Histone H3 antibody - Nuclear Loading Control (ab21054) at 1/5000 dilution
Lane 1 :NIH/3T3 whole cell lysate (ab7179) at 10 µg
Lane 2 : Yeast S.cerevisiae Whole Cell Lysate at 10 µg
Lane 3 : Drosophila Embryo Nuclear Extract at 20 µg
Performed under reducing conditions.
Predicted band size: 15.4 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Histone H3 antibody - Nuclear Loading Control (ab21054)IHC image of Histone H3 staining in a section of formalin-fixed paraffin-embedded normal human cervix tissue*, performed on a Leica BONDTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab21054, 1/500 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Histone H3 antibody - Nuclear Loading Control (ab21054)IHC image of Histone H3 staining in human skin formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab21054, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
