Recombinant human PDGF B protein (Active) (ab259425)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies, HPLC, MS, Cell Culture
Preparation and Storage
-
Alternative names
- Becaplermin
- c sis
- FLJ12858
see all - Information by UniProt
Images
-
Functional Studies - Recombinant Human PDGF B protein (ab259425)
Fully biologically active compared to a standard. The ED50 is is 0.9388 ng/mL corresponding to a Specific Activity of 1.07 x 106 IU/mg.
-
SDS-PAGE - Recombinant Human PDGF B protein (ab259425)SDS-PAGE analysis of ab259425.
-
HPLC - Recombinant Human PDGF B protein (ab259425)Purity 98%.
The spectrum was recorded using a 1260 Infinity II HPLC system with DAD and a MabPac RP column (3.0x100 mm, 4 µm). 5 µL of purified protein was injected and the gradient run from 80 % water:TFA (99.9:0.1 v/v) and 20 % acetonitrile:water:TFA (90:9.9:0.1 v/v/v) to 20 % water:TFA (99.9:0.1 v/v) and 80 % acetonitrile:water:TFA (90:9.9:0.1 v/v/v) within 3 minutes followed by an isocratic step for another 3 min. Flow rate was 0.5 mL/min and the column compartment temperature was 50 °C.
-
Mass Spectrometry - Recombinant Human PDGF B protein (ab259425)M + 0.41 Da (Calc. mass 12351.59 ).
The spectrum was recorded with a 6545XT AdvanceBio LC/Q-TOF (Agilent Technologies) and a MabPac RP column (42.1x50 mm, 4 µm, Thermo Scientific). 5 µL of purified protein was injected and the gradient run from 85 % water:FA (99.9:0.1 v/v) and 15 % acetonitrile:FA (90:9.9:0.1 v/v/v) to 55 % water:FA (99.9:0.1 v/v) and 45 % acetonitrile:FA (90:9.9:0.1 v/v/v) within 3 minutes followed by an isocratic step for another 2.5 min. Flow rate was 0.4 mL/min and the column compartment temperature was 60 °C. Data was analysed and deconvoluted using the Bioconfirm software (Agilent Technologies).