Anti-Ly6g antibody [EPR22909-135] (ab238132)
![Anti-Ly6g antibody [EPR22909-135] (ab238132)](https://www.abcam.com/ps/products/238/ab238132/Images/ab238132-1-anti-ly6g-antibody-epr22909-135-immunohistochemistry-spleen-mouse.jpg "Anti-Ly6g antibody [EPR22909-135] (ab238132)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22909-135] to Ly6g
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Ly6g antibody [EPR22909-135]
See all Ly6g primary antibodies -
Description
Rabbit monoclonal [EPR22909-135] to Ly6g -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt MouseHumanIHC-P Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Mouse spleen and lung tissues. Flow Cyt: Mouse peripheral blood mononuclear cell and HEK-293T cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22909-135 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly6g antibody [EPR22909-135] (ab238132)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Ly6g with ab238132 at 1/2000 dilution (0.32 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on neutrophils in mouse spleen (PMID: 21976773) is observed. The section was incubated with ab238132 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly6g antibody [EPR22909-135] (ab238132)](https://www.abcam.com/ps/products/238/ab238132/Images/ab238132-2-anti-ly6g-antibody-epr22909-135-immunohistochemistry-lung-mouse.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly6g antibody [EPR22909-135] (ab238132)Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Ly6g with ab238132 at 1/2000 dilution (0.32 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on neutrophils in mouse lung (PMID: 22434870) is observed. The section was incubated with ab238132 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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![Flow Cytometry - Anti-Ly6g antibody [EPR22909-135] (ab238132)](https://www.abcam.com/ps/products/238/ab238132/Images/ab238132-3-anti-ly6g-antibody-epr22909-135-flowcytometry-pbmc-mouse.jpg)
Flow Cytometry - Anti-Ly6g antibody [EPR22909-135] (ab238132)Flow cytometric analysis of mouse peripheral blood mononuclear cell (PBMC) cells labeling Ly6g with ab238132 at 1/600 dilution. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Cells were stained with isotype control rabbit monoclonal IgG ab172730 (Left) or ab238132 (Right), then stained with anti-CD11b conjugated to BV421.
Gated on viable cells. -
![Flow Cytometry - Anti-Ly6g antibody [EPR22909-135] (ab238132)](https://www.abcam.com/ps/products/238/ab238132/Images/ab238132-4-anti-ly6g-antibody-epr22909-135-flowcytometry-293t-overexpression-mouse.jpg)
Flow Cytometry - Anti-Ly6g antibody [EPR22909-135] (ab238132)Flow cytometric analysis of HEK-293T (Human embryonic kidney epithelial cell) transfected with HA-tagged Ly6c overexpression construct (Left) / HEK-293T transfected with HA-tagged Ly6g overexpression construct (Right) cells, labeling Ly6g with ab238132 at 1/600 dilution. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Ly6c (Left) and Ly6g (Right) overexpression cells were surface stained with ab238132 and anti-HA tag conjugated to Alexa Fluor®647.
ab238132 does not cross-react with Ly6c.
Gated on viable cells.
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![Anti-Ly6g antibody [EPR22909-135] (ab238132)](https://www.abcam.com/ps/products/238/ab238132/Images/ab238132-5-benefits-of-recombinant-antibodies.png)
Anti-Ly6g antibody [EPR22909-135] (ab238132)
