Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PD1 antibody [NAT105] - Chimeric – BSA and Azide free (ab233091)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [NAT105] to PD1 - Chimeric – BSA and Azide free
  • Suitable for: WB, IP, Flow Cyt, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-PD1 antibody [NAT105] - Chimeric – BSA and Azide free
    See all PD1 primary antibodies

  • Description

    Rabbit monoclonal [NAT105] to PD1 - Chimeric – BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, Flow Cyt, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Tissue, cells or virus corresponding to Human PD1. Specifically, human YT cells (NK-like leukemia cell line) that express PD1.
    Database link: Q15116

  • Positive control

    • IHC-P: Human tonsil tissue. Flow Cyt: MOLT-4 cells treated with 500 ng/ml Ionomycin and 10 ng/ml Phorbol-12-myristate-13-acetate (PMA) for 24 hours.

  • General notes

    ab233091 is the carrier-free version of ab216352 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab233091 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This rabbit antibody has been engineered from the mouse monoclonal parent antibody Anti-PD1 antibody [NAT105] (ab52587). By necessity, some mouse sequence is retained as part of the variable domain. We recommend using an Fc-directed, preadsorbed secondary antibody if multiplexing with mouse monoclonals. For example, ab150097 (AF488), ab150100 (AF594), ab150099 (AF647) or ab98467 (HRP).

Properties

Images

  • Flow Cytometry - Anti-PD1 antibody [NAT105-EPR21106] - Chimeric – BSA and Azide free (ab233091)
    Flow Cytometry - Anti-PD1 antibody [NAT105-EPR21106] - Chimeric – BSA and Azide free (ab233091)

    Flow cytometric analysis of MOLT-4 (human lymphoblastic leukemia cell line) cell line treated with 500 ng/ml Ionomycin and 10 ng/ml Phorbol-12-myristate-13-acetate (PMA) for 24 hours (Red) or Untreated control (Green) labeling PD1 with ab216352 at 1/600 dilution compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216352).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105-EPR21106] - Chimeric – BSA and Azide free (ab233091)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105-EPR21106] - Chimeric – BSA and Azide free (ab233091)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling PD1 with ab216352 at 1/50 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use. Positive staining on T cells of human tonsil germinal center (PMID: 20087161; PMID: 17640856). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216352).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Anti-PD1 antibody [NAT105] - Chimeric – BSA and Azide free (ab233091)
    Anti-PD1 antibody [NAT105] - Chimeric – BSA and Azide free (ab233091)

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