Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BTK (phospho Y551) antibody [BtkY551-G12] (ab278611)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [BtkY551-G12] to BTK (phospho Y551)
  • Suitable for: ELISA, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-BTK (phospho Y551) antibody [BtkY551-G12]
    See all BTK primary antibodies

  • Description

    Rabbit monoclonal [BtkY551-G12] to BTK (phospho Y551)

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ELISA
    Human
    Flow Cyt
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human BTK (phospho Y551). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q06187

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Flow cyt: Ramos cells (treated with pervanadate).

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: 49.88% PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
  • Concentration information loading...

  • Purity

    Protein A/G purified

  • Clonality

    Monoclonal

  • Clone number

    BtkY551-G12

  • Isotype

    IgG

  • Light chain type

    kappa

  • Research areas

Images

  • Flow Cytometry - Anti-BTK (phospho Y551) antibody [BtkY551-G12] (ab278611)
    Flow Cytometry - Anti-BTK (phospho Y551) antibody [BtkY551-G12] (ab278611)
    Flow cytometric analysis of Ramos cells secondary antibody only negative control (blue) or treated with imatinib (red) or with pervanadate (green) using ab278611 at 0.1 µg/mL.
  • ELISA - Anti-BTK (phospho Y551) antibody [BtkY551-G12] (ab278611)
    ELISA - Anti-BTK (phospho Y551) antibody [BtkY551-G12] (ab278611)
    Direct ELISA using Ramos cellular lysate coated directly to the plate surface after lysis following no treatment or treatment with 1mM pervanadate for 30 min. ELISA wells were tested in duplicate using 0.25 mg/mL total protein coated lysate and 1 ug/mL ab278611.
  • ELISA - Anti-BTK (phospho Y551) antibody [BtkY551-G12] (ab278611)
    ELISA - Anti-BTK (phospho Y551) antibody [BtkY551-G12] (ab278611)
    Peptide ELISA using ab278611 titered starting from 0.3 ug/mL shows binding to only Btk pY551 phospho peptide and no cross-reactivity to Btk Y551 non-phospho peptide or to control peptides.
  • Anti-BTK (phospho Y551) antibody [BtkY551-G12] (ab278611)
    Anti-BTK (phospho Y551) antibody [BtkY551-G12] (ab278611)

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