Anti-ATP5H antibody [7F9BG1] (ab110275)
![Anti-ATP5H antibody [7F9BG1] (ab110275)](https://www.abcam.com/ps/products/110/ab110275/Images/ab110275-172342-anti-atp5h-antibody-7f9bg1-western-blot.jpg "Anti-ATP5H antibody [7F9BG1] (ab110275)")
Key features and details
- Mouse monoclonal [7F9BG1] to ATP5H
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Mouse, Rat, Cow, Human, African green monkey
- Isotype: IgG2b
Overview
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Product name
Anti-ATP5H antibody [7F9BG1]
See all ATP5H primary antibodies -
Description
Mouse monoclonal [7F9BG1] to ATP5H -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanWB Human
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Immunogen
Other Immunogen Type. This information is considered to be commercially sensitive.
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Positive control
- Isolated mitochondria from Human heart, Bovine heart, Rat heart, Mouse heart, and HepG2, Cultured Human embryonic lung-derived fibroblasts (strain MRC5), HeLa cells
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General notes
This antibody clone is manufactured by Abcam.
For mouse and rat samples, this antibody will only recognize ATP5H in purified mitochondrial samples. Mouse and rat cell lysates and tissue homogenates are not recommneded with this antibody.
Product was previously marketed under the MitoSciences sub-brand.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Near homogeneity as judged by SDS-PAGE. ab110275 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
7F9BG1 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-ATP5H antibody [7F9BG1] (ab110275)All lanes : Anti-ATP5H antibody [7F9BG1] (ab110275) at 1 µg/ml
Lane 1 : HDFn (human) cell lysates at 20 µg
Lane 2 : COS-7 (African green monkey kidney fibroblast-like cell line) cell lysates at 20 µg
Lane 3 : H4IIE (rat) cell lysates at 20 µg
Lane 4 : MEF (mouse) cell lysates at 20 µg
Lane 5 : bovine heart mitochondria lysates at 5 µg
Predicted band size: 18 kDa
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![Western blot - Anti-ATP5H antibody [7F9BG1] (ab110275)](https://www.abcam.com/ps/products/110/ab110275/Images/ab110275-123472-anti-atp5h-antibody-7f9bg1-western-blot.jpg)
Western blot - Anti-ATP5H antibody [7F9BG1] (ab110275)All lanes : Anti-ATP5H antibody [7F9BG1] (ab110275) at 1/1 dilution
Lane 1 : Human heart mitochondria at 10 µg
Lane 2 : Bovine heart mitochondria at 4 µg
Lane 3 : Rat heart mitochondria at 10 µg
Lane 4 : Mouse heart mitochondria at 10 µg
Lane 5 : HepG2 mitochondria at 20 µg
Predicted band size: 18 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-ATP5H antibody [7F9BG1] (ab110275)](https://www.abcam.com/ps/products/110/ab110275/Images/ab110275-123471-anti-atp5h-antibody-7f9bg1-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ATP5H antibody [7F9BG1] (ab110275)Mitochondrial localization of ATP5H. Cultured Human embryonic lung-derived fibroblasts (strain MRC5) were fixed, permeabilized and then labeled with ab110275 (5 µg/ml) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG(H+L) secondary antibody (2 µg/ml). -
![Flow Cytometry - Anti-ATP5H antibody [7F9BG1] (ab110275)](https://www.abcam.com/ps/products/110/ab110275/Images/ab110275-123470-anti-atp5h-antibody-7f9bg1-flow-cytometry.jpg)
Flow Cytometry - Anti-ATP5H antibody [7F9BG1] (ab110275)HeLa cells were stained with 1 µg/mL ab110275 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
