Anti-ATP5H antibody (ab230101)
Key features and details
- Rabbit polyclonal to ATP5H
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ATP5H antibody
See all ATP5H primary antibodies -
Description
Rabbit polyclonal to ATP5H -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length protein corresponding to Human ATP5H aa 1 to the C-terminus.
Database link: O75947 -
Positive control
- WB: Mouse kidney, liver and brain lysates; HeLa, HepG2 and PC-3 whole cell lysates. IHC-P: Human brain and small intestine tissues.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ATP5H antibody (ab230101)All lanes : Anti-ATP5H antibody (ab230101) at 1/1000 dilution
Lane 1 : Mouse kidney lysate
Lane 2 : Mouse liver lysate
Lane 3 : Mouse brain lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 5 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 6 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
Lane 7 : U-251 MG (human brain glioma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 16,18 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5H antibody (ab230101)Paraffin-embedded human brain tissue stained for ATP5H using ab230101 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5H antibody (ab230101)Paraffin-embedded human small intestine tissue stained for ATP5H using ab230101 at 1/100 dilution in immunohistochemical analysis.
