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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-DDX5 antibody (ab245472)

Anti-DDX5 antibody (ab245472)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to DDX5
  • Suitable for: IP, WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-DDX5 antibody
    See all DDX5 primary antibodies
  • Description

    Rabbit polyclonal to DDX5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Orangutan
  • Immunogen

    Synthetic peptide within Human DDX5 aa 575-614. The exact sequence is proprietary. NP_004387.1
    Database link: P17844

  • Positive control

    • WB: HeLa, HEK-293T and NIH/3T3 whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Human breast carcinoma tissue; Mouse renal cell carcinoma tissue.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: Tris buffered saline, 0.1% BSA

    pH 7-8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab245472 was affinity purified using an epitope specific to DDX5 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Other

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX5 antibody (ab245472)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX5 antibody (ab245472)

    Formalin-fixed, paraffin-embedded mouse renal cell carcinoma tissue stained for DDX5 using ab245472 at 1/200 dilution in immunohistochemical analysis. DAB staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX5 antibody (ab245472)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX5 antibody (ab245472)

    Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for DDX5 using ab245472 at 1/200 dilution in immunohistochemical analysis. DAB staining.

  • Immunoprecipitation - Anti-DDX5 antibody (ab245472)
    Immunoprecipitation - Anti-DDX5 antibody (ab245472)

    DDX5 was immunoprecipitated from 1 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate using ab245472 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab245472 at 1 µg/ml.

    Lane 1: ab245472 IP in HeLa whole cell lysate.
    Lane 2: Control IgG in HeLa whole cell lysate.

    Detection: Chemiluminescence with an exposure time of 1 second.

    Prepared using NETN lysis buffer.

  • Western blot - Anti-DDX5 antibody (ab245472)
    Western blot - Anti-DDX5 antibody (ab245472)
    All lanes : Anti-DDX5 antibody (ab245472) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Exposure time: 1 second


    Prepared using NETN lysis buffer.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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