Anti-CD55 antibody [EPR22362-255] - BSA and Azide free (ab243560)
![Anti-CD55 antibody [EPR22362-255] - BSA and Azide free (ab243560)](https://www.abcam.com/ps/products/243/ab243560/Images/ab243560-3-243231FC2.jpg "Anti-CD55 antibody [EPR22362-255] - BSA and Azide free (ab243560)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22362-255] to CD55 - BSA and Azide free
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-CD55 antibody [EPR22362-255] - BSA and Azide free
See all CD55 primary antibodies -
Description
Rabbit monoclonal [EPR22362-255] to CD55 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or WB -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: Human peripheral blood mononuclear cells. Flow cyt: Human peripheral blood mononuclear cells.
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General notes
Ab243560 is the carrier-free version of ab243231. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab243560 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22362-255 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-CD55 antibody [EPR22362-255] - BSA and Azide free (ab243560)
Flow cytometric analysis of IMR-32 (human neuroblastoma neuroblast cell line) cells labeling CD55 with ab243231 at 1/500 (Right panel) compared with Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Left panel). Goat anti rabbit IgG (Dylight ® 488), at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
Negative control: IMR-32 (PMID: 27043658 and 15699172).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243231).
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![Flow Cytometry - Anti-CD55 antibody [EPR22362-255] - BSA and Azide free (ab243560)](https://www.abcam.com/ps/products/243/ab243560/Images/ab243560-2-243231FC1.jpg)
Flow Cytometry - Anti-CD55 antibody [EPR22362-255] - BSA and Azide free (ab243560)Flow cytometric analysis of human peripheral blood mononuclear cells labeling CD55 with ab243231 at 1/500 (Right panel) compared with Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Left panel). Goat anti rabbit IgG (Dylight ® 488), at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
Relative levels of CD55 expression on monocytes (SSC high population) and lymphocyte (main population, SSC low) subsets was described in the literature (PMID: 16999828).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243231).
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![Immunocytochemistry/ Immunofluorescence - Anti-CD55 antibody [EPR22362-255] - BSA and Azide free (ab243560)](https://www.abcam.com/ps/products/243/ab243560/Images/ab243560-1-243231ICC1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD55 antibody [EPR22362-255] - BSA and Azide free (ab243560)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized human peripheral blood mononuclear cells labeling CD55 with ab243231 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing Membranous staining in human PBMC cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution.
Control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
Negative control: IMR-32 (PMID: 27043658).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243231).
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![Anti-CD55 antibody [EPR22362-255] - BSA and Azide free (ab243560)](https://www.abcam.com/ps/products/243/ab243560/Images/ab243560-4-benefits-of-recombinant-antibodies.png)
Anti-CD55 antibody [EPR22362-255] - BSA and Azide free (ab243560)
