Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD55 antibody [EPR22362-255] - BSA and Azide free (ab243560)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22362-255] to CD55 - BSA and Azide free
  • Suitable for: ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-CD55 antibody [EPR22362-255] - BSA and Azide free
    See all CD55 primary antibodies

  • Description

    Rabbit monoclonal [EPR22362-255] to CD55 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or WB

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: Human peripheral blood mononuclear cells. Flow cyt: Human peripheral blood mononuclear cells.

  • General notes

    Ab243560 is the carrier-free version of ab243231. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab243560 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22362-255

  • Isotype

    IgG

  • Research areas

Images

  • Flow Cytometry - Anti-CD55 antibody [EPR22362-255] - BSA and Azide free (ab243560)
    Flow Cytometry - Anti-CD55 antibody [EPR22362-255] - BSA and Azide free (ab243560)

    Flow cytometric analysis of IMR-32 (human neuroblastoma neuroblast cell line) cells  labeling CD55 with ab243231 at 1/500 (Right panel) compared with Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Left panel). Goat anti rabbit IgG (Dylight ® 488), at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

    Negative control: IMR-32 (PMID: 27043658 and 15699172).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243231).

  • Flow Cytometry - Anti-CD55 antibody [EPR22362-255] - BSA and Azide free (ab243560)
    Flow Cytometry - Anti-CD55 antibody [EPR22362-255] - BSA and Azide free (ab243560)

    Flow cytometric analysis of human peripheral blood mononuclear cells labeling CD55 with ab243231 at 1/500 (Right panel) compared with Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Left panel). Goat anti rabbit IgG (Dylight ® 488), at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

    Relative levels of CD55 expression on monocytes (SSC high population) and lymphocyte (main population, SSC low) subsets was described in the literature (PMID: 16999828).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243231).

  • Immunocytochemistry/ Immunofluorescence - Anti-CD55 antibody [EPR22362-255] - BSA and Azide free (ab243560)
    Immunocytochemistry/ Immunofluorescence - Anti-CD55 antibody [EPR22362-255] - BSA and Azide free (ab243560)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized human peripheral blood mononuclear cells labeling CD55 with ab243231 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing Membranous staining in human PBMC cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution. 

    Control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    Negative control: IMR-32 (PMID: 27043658).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243231).

  • Anti-CD55 antibody [EPR22362-255] - BSA and Azide free (ab243560)
    Anti-CD55 antibody [EPR22362-255] - BSA and Azide free (ab243560)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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