Recombinant human TTK/Mps1 protein (ab89589)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, WB, Functional Studies
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Product name
Recombinant human TTK/Mps1 protein -
Biological activity
The Specific activity of ab89589 was determined to be 19 nmol/min/mg. -
Expression system
Baculovirus infected Sf9 cells -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Tags
GST tag N-Terminus
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Preparation and Storage
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Applications
SDS-PAGE
Western blot
Functional Studies
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Form
Liquid -
Additional notes
ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
Images
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Functional Studies - Recombinant human TTK/Mps1 protein (ab89589)The specific activity of TTK/Mps1 (ab89589) was determined to be 18 nmol/min/mg as per activity assay protocol
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SDS-PAGE - Recombinant human TTK/Mps1 protein (ab89589)SDS PAGE analysis of ab89589 -
SDS-PAGE - Recombinant human TTK/Mps1 protein (ab89589)SDS PAGE analysis of ab89589 -
SDS-PAGE - Recombinant human TTK/Mps1 protein (ab89589)SDS-PAGE showing ab89589 at approximately 130kDa. -
Functional Studies - Recombinant human TTK/Mps1 protein (ab89589)Kinase Assay demonstrating specific activity of ab89589. -
Western blot - Recombinant human TTK/Mps1 protein (ab89589)Anti-TTK/Mps1 antibody [N1] - BSA free (ab11108) at 10 µg/ml +Recombinant human TTK/Mps1 protein (ab89589) at 0.01 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 8 minutesab11108 recognizes the full length tagged recombinant TTK/Mps1 protein (ab89589) which has an expected molecular weight of 130 kDa.