Recombinant human TTK/Mps1 protein (ab89589)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, WB, Functional Studies
-
Product name
Recombinant human TTK/Mps1 protein -
Biological activity
The Specific activity of ab89589 was determined to be 19 nmol/min/mg. -
Expression system
Baculovirus infected Sf9 cells -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Tags
GST tag N-Terminus
-
Preparation and Storage
-
Applications
SDS-PAGE
Western blot
Functional Studies
-
Form
Liquid -
Additional notes
ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
-
Concentration information loading...
Images
-
Functional Studies - Recombinant human TTK/Mps1 protein (ab89589)The specific activity of TTK/Mps1 (ab89589) was determined to be 18 nmol/min/mg as per activity assay protocol
-
SDS-PAGE - Recombinant human TTK/Mps1 protein (ab89589)SDS PAGE analysis of ab89589 -
SDS-PAGE - Recombinant human TTK/Mps1 protein (ab89589)SDS PAGE analysis of ab89589 -
SDS-PAGE - Recombinant human TTK/Mps1 protein (ab89589)SDS-PAGE showing ab89589 at approximately 130kDa. -
Functional Studies - Recombinant human TTK/Mps1 protein (ab89589)Kinase Assay demonstrating specific activity of ab89589. -
Western blot - Recombinant human TTK/Mps1 protein (ab89589)Anti-TTK/Mps1 antibody [N1] - BSA free (ab11108) at 10 µg/ml +Recombinant human TTK/Mps1 protein (ab89589) at 0.01 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 8 minutesab11108 recognizes the full length tagged recombinant TTK/Mps1 protein (ab89589) which has an expected molecular weight of 130 kDa.
