Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Mouse monoclonal [OTI1G3] to SIRT6
Suitable for: WB, ICC/IF
Reacts with: Mouse, Dog, Human, African green monkey
Isotype: IgG1

Product name

Anti-SIRT6 antibody [OTI1G3]
See all SIRT6 primary antibodies
Description

Mouse monoclonal [OTI1G3] to SIRT6
Host species

Mouse
Tested applications

Suitable for: WB, ICC/IFmore details
Species reactivity

Reacts with: Mouse, Dog, Human, African green monkey
Immunogen

Recombinant fragment corresponding to Human SIRT6 aa 50 to the C-terminus. Produced in E.coli (NP_057623).
Database link: Q8N6T7

Run BLAST with Run BLAST with
Positive control
- WB: HEK-293T cell lysate transfected with pCMV6-ENTRY SIRT6 cDNA; HepG2, HeLa, COS-7, Jurkat, MDCK and MCF7 cell extracts. ICC/IF: COS-7 cells transiently transfected by pCMV6-ENTRY SIRT6.
General notes

Clone OTI1G3 (formerly 1G3).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA
Concentration information loading...
Purity

Affinity purified
Purification notes

Purified from cell culture supernatant by affinity chromatography.
Clonality

Monoclonal
Clone number

OTI1G3
Isotype

IgG1
Research areas

Western blot - Anti-SIRT6 antibody [OTI1G3] (ab119007)

All lanes : Anti-SIRT6 antibody [OTI1G3] (ab119007) at 1/200 dilution

Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell extract
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 3 : SVT2 (Mouse) cell extract
Lane 4 : A549 (human lung carcinoma cell line) cell extract
Lane 5 : COS-7 (african green monkey kidney fibroblast-like cell line) cell extract
Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) cell extract
Lane 7 : MDCK (canine kidney cell line) cell extract
Lane 8 : PC-12 (rat adrenal gland pheochromocytoma cell line) cell extract
Lane 9 : MCF7 (human breast adenocarcinoma cell line) cell extract

Lysates/proteins at 35 µg per lane.

Predicted band size: 39 kDa
Western blot - Anti-SIRT6 antibody [OTI1G3] (ab119007)

All lanes : Anti-SIRT6 antibody [OTI1G3] (ab119007) at 1/500 dilution

Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY SIRT6 cDNA

Lysates/proteins at 5 µg per lane.

Predicted band size: 39 kDa
Immunocytochemistry/ Immunofluorescence - Anti-SIRT6 antibody [OTI1G3] (ab119007)

COS-7 (african green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV6-ENTRY SIRT6 stained for SIRT6 (green) using ab119007 at 1/100 dilution in ICC/IF.

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