Anti-TAZ (phospho S311) antibody [14HCLC] (ab277791)
![Anti-TAZ (phospho S311) antibody [14HCLC] (ab277791)](https://www.abcam.com/ps/products/277/ab277791/Images/ab277791-1-antiPhosphoWWTR1S311recombinantpolyclonalantibody14hclcIF.jpg "Anti-TAZ (phospho S311) antibody [14HCLC] (ab277791)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit recombinant multiclonal [14HCLC] to TAZ (phospho S311)
- Suitable for: ICC
- Reacts with: Human
Overview
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Product name
Anti-TAZ (phospho S311) antibody [14HCLC]
See all TAZ primary antibodies -
Description
Rabbit recombinant multiclonal [14HCLC] to TAZ (phospho S311) -
Host species
Rabbit -
Tested applications
Suitable for: ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide aa 307-314 (phospho S311).
Database link: Q9GZV5 -
Positive control
- ICC: HeLa cells
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General notes
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.
Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituent: 99.91% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Recombinant Multiclonal -
Clone number
14HCLC -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry - Anti-TAZ antibody (ab277791)
For immunofluorescence analysis, HeLa cells were fixed and permeabilized for detection of endogenous WWTR1 pS311 using Anti-Phospho-WWTR1 (Ser311) Recombinant Rabbit Multiclonal Antibody (ab277791, 1/100) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (1/2000). Nuclei (blue) were stained using ProLong Diamond Antifade Mountant with DAPI(Product # P36962) and cytoskeletal F-actin (red) staining using Rhodamine Phalloidin (1/300) Panel a-d) shows representative un-treated cells that were stained for detection and localization of Phospho-WWTR1 (Ser311) protein (green) with no signal compared to panel e-h) clearly demonstrating enhanced cytoplasmic localisation of WWTR1 pS311 in HeLa cells treated with Okadaic acid (1 M, 1h) and MG132 (40 M, 3h). The images were captured at 60X magnification.
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![Anti-TAZ antibody [14HCLC] (ab277791)](https://www.abcam.com/ps/products/277/ab277791/Images/ab277791-2-benefits-of-recombinant-antibodies.jpg)
Anti-TAZ antibody [14HCLC] (ab277791)