Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD147 antibody [EPR18008-8] - BSA and Azide free (ab222389)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18008-8] to CD147 - BSA and Azide free
  • Suitable for: Sandwich ELISA, WB, IHC-P, Flow Cyt, IP, ICC/IF
  • Reacts with: Mouse, Recombinant fragment

Overview

  • Product name

    Anti-CD147 antibody [EPR18008-8] - BSA and Azide free
    See all CD147 primary antibodies

  • Description

    Rabbit monoclonal [EPR18008-8] to CD147 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Sandwich ELISA, WB, IHC-P, Flow Cyt, IP, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Recombinant fragment

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse CD147 recombinant protein fragment; RAW 264.7, WEHI-3 and bEND.3 whole cell lysates; mouse liver lysate. IHC-P: Mouse intestine tissue. ICC/IF: WEHI-231 and bEND.3 cells. Flow Cyt: Mouse thymocytes. IP: RAW 264.7 whole cell lysate.

  • General notes

    ab222389 is the carrier-free version of ab188190.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD147 antibody [EPR18008-8] - BSA and Azide free (ab222389)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD147 antibody [EPR18008-8] - BSA and Azide free (ab222389)

    Immunohistochemical analysis of paraffin-embedded mouse intestine tissue labeling CD147 with ab188190 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membrane staining on mouse intestine is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188190).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD147 antibody [EPR18008-8] - BSA and Azide free (ab222389)
    Immunocytochemistry/ Immunofluorescence - Anti-CD147 antibody [EPR18008-8] - BSA and Azide free (ab222389)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized bEND.3 (Mouse brain capillary endothelial cell line) cells labeling CD147 with ab188190 at 1/250 dilution, followed by by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cell membrane staining on bEND.3 cell line. The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab188190 at 1/250 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution.
    -ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188190).

  • Flow Cytometry - Anti-CD147 antibody [EPR18008-8] - BSA and Azide free (ab222389)
    Flow Cytometry - Anti-CD147 antibody [EPR18008-8] - BSA and Azide free (ab222389)

    Flow cytometric analysis of fresh mouse thymocytes labeling CD147 with ab188190 at 1/200 dilution (red) compared with Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488) at 1/500 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188190).

  • Sandwich ELISA - Anti-CD147 antibody [EPR18008-8] - BSA and Azide free (ab222389)
    Sandwich ELISA - Anti-CD147 antibody [EPR18008-8] - BSA and Azide free (ab222389)

    Standard Curve for CD147 (Analyte: Recombinant mouse CD147 protein) dilution range 0 pg/mL to 500 pg/mL using Capture antibody at 0.2 ug/mL and Detector Antibody at 0.5 ug/mL. Secondary antibody: Peroxidase Streptavidin SA-HRP at 1/20000 dilution. Concentration of ab188190 may vary from lot to lot; please use this curve as guideline.

    Washing buffer: 1X PBST
    Blocking/Diluting buffer and concentration: 1% BSA/PBS

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188190).

  • Immunocytochemistry/ Immunofluorescence - Anti-CD147 antibody [EPR18008-8] - BSA and Azide free (ab222389)
    Immunocytochemistry/ Immunofluorescence - Anti-CD147 antibody [EPR18008-8] - BSA and Azide free (ab222389)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized WEHI-231 (Mouse B Cell Lymphoma cell line) cells labeling CD147 with ab188190 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cell membrane staining on WEHI-231 cell line. The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab188190 at 1/250 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution.
    -ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188190).

  • Immunoprecipitation - Anti-CD147 antibody [EPR18008-8] - BSA and Azide free (ab222389)
    Immunoprecipitation - Anti-CD147 antibody [EPR18008-8] - BSA and Azide free (ab222389)

    CD147 was immunoprecipitated from 1 mg of RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate with ab188190 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab188190 at 1/5000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: RAW 264.7 whole cell lysate 10µg (Input).

    Lane 2: ab188190 IP in RAW 264.7 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab188190 in RAW 264.7 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188190).

  • Anti-CD147 antibody [EPR18008-8] - BSA and Azide free (ab222389)
    Anti-CD147 antibody [EPR18008-8] - BSA and Azide free (ab222389)

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