Human IL-13 RA1 ELISA Kit (ab267620)
Key features and details
- Sensitivity: 0.042 ng/ml
- Range: 0.041 ng/ml - 10 ng/ml
- Sample type: Cell culture supernatant, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IL-13 RA1 ELISA Kit
See all IL-13 receptor alpha 1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.042 ng/ml -
Range
0.041 ng/ml - 10 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 78.21 70% - 84% Cell culture media 72.33 69% - 79% EDTA Plasma 76.24 74% - 78% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human IL-13 RA1 ELISA Kit is designed for the quantitative determination of IL-13 RA1 in cell culture supernatants, plasma and serum samples.
Δ Note: Collect plasma using EDTA as an anticoagulant. Heparin and citrate are not recommended as anticoagulants for use in this assay.
This assay employs an antibody specific for human IL-13 RA1 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-13 RA1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-13 RA1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-13 RA1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA kit shows no cross-reactivity with the following cytokines tested: Human Angiogenin, BDNF Activin A, AgRP, Angiogenin, Angiopoietin-1, Angiostatin, Cathepsin S, CD40 (TNFRSF5), Cripto-1, DAN, DKK-1, ECadherin, Fas Ligand (TNFSF6), Fc gamma RIIB/C (CD32B/C), Follistatin, Galectin-7, gp130, ICAM-2 (CD102), IL-1 R4 (ST2), IL-13 R alpha 2, IL-17B, IL-2 R alpha, IL-2 R beta (CD122), IL-23, LAP/TGF beta 1, NrCAM, PAI 1, PDGF-AB, Resistin, SDF-1 beta (CXCL12 beta), Siglec-5 (CD170), Sonic Hedgehog NTerminal (Shh-N), TGF beta 2, Thrombopoietin (TPO), Tie-2, TRAIL R4 (TNFRSF10D), TREM-1, TROP1 (EpCAM), VEGF-C, VEGFR1.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Anti-Human IL-13 RA1 coated Microplate (12 x 8 wells) 1 unit Assay Diluent C 1 x 30ml Biotinylated Anti-Human IL-13 RA1 Detection Antibody 2 vials Human IL-13 RA1 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Binds with low affinity to interleukin-13 (IL13). Together with IL4RA can form a functional receptor for IL13. Also serves as an alternate accessory protein to the common cytokine receptor gamma chain for interleukin-4 (IL4) signaling, but cannot replace the function of IL2RG in allowing enhanced interleukin-2 (IL2) binding activity. -
Tissue specificity
Ubiquitous. Highest levels in heart, liver, skeletal muscle and ovary; lowest levels in brain, lung and kidney. Also found in B-cells, T-cells and endothelial cells. -
Sequence similarities
Belongs to the type I cytokine receptor family. Type 5 subfamily. -
Domain
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- bB128O4.2.1 (interleukin 13 receptor, alpha 1)
- Cancer/testis antigen 19
- CD213a1
see all -
Database links
- Entrez Gene: 3597 Human
- Omim: 300119 Human
- SwissProt: P78552 Human
- Unigene: 496646 Human
Images
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Example data
This standard curve is for demonstration only. A standard curve must be run with each assay.
