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Human IL-13 RA1 ELISA Kit (ab267620)

Key features and details

  • Sensitivity: 0.042 ng/ml
  • Range: 0.041 ng/ml - 10 ng/ml
  • Sample type: Cell culture supernatant, EDTA Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human IL-13 RA1 ELISA Kit
    See all IL-13 receptor alpha 1 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall
    Inter-assay
    Sample n Mean SD CV%
    Overall

  • Sample type

    Cell culture supernatant, Serum, EDTA Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    0.042 ng/ml

  • Range

    0.041 ng/ml - 10 ng/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 78.21 70% - 84%
    Cell culture media 72.33 69% - 79%
    EDTA Plasma 76.24 74% - 78%

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human IL-13 RA1 ELISA Kit is designed for the quantitative determination of IL-13 RA1 in cell culture supernatants, plasma and serum samples.


    Δ Note: Collect plasma using EDTA as an anticoagulant. Heparin and citrate are not recommended as anticoagulants for use in this assay.


    This assay employs an antibody specific for human IL-13 RA1 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-13 RA1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-13 RA1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-13 RA1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    This ELISA kit shows no cross-reactivity with the following cytokines tested: Human Angiogenin, BDNF Activin A, AgRP, Angiogenin, Angiopoietin-1, Angiostatin, Cathepsin S, CD40 (TNFRSF5), Cripto-1, DAN, DKK-1, ECadherin, Fas Ligand (TNFSF6), Fc gamma RIIB/C (CD32B/C), Follistatin, Galectin-7, gp130, ICAM-2 (CD102), IL-1 R4 (ST2), IL-13 R alpha 2, IL-17B, IL-2 R alpha, IL-2 R beta (CD122), IL-23, LAP/TGF beta 1, NrCAM, PAI 1, PDGF-AB, Resistin, SDF-1 beta (CXCL12 beta), Siglec-5 (CD170), Sonic Hedgehog NTerminal (Shh-N), TGF beta 2, Thrombopoietin (TPO), Tie-2, TRAIL R4 (TNFRSF10D), TREM-1, TROP1 (EpCAM), VEGF-C, VEGFR1.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Anti-Human IL-13 RA1 coated Microplate (12 x 8 wells) 1 unit
    Assay Diluent C 1 x 30ml
    Biotinylated Anti-Human IL-13 RA1 Detection Antibody 2 vials
    Human IL-13 RA1 Standard (Lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Solution 1 x 12ml

  • Research areas

  • Function

    Binds with low affinity to interleukin-13 (IL13). Together with IL4RA can form a functional receptor for IL13. Also serves as an alternate accessory protein to the common cytokine receptor gamma chain for interleukin-4 (IL4) signaling, but cannot replace the function of IL2RG in allowing enhanced interleukin-2 (IL2) binding activity.

  • Tissue specificity

    Ubiquitous. Highest levels in heart, liver, skeletal muscle and ovary; lowest levels in brain, lung and kidney. Also found in B-cells, T-cells and endothelial cells.

  • Sequence similarities

    Belongs to the type I cytokine receptor family. Type 5 subfamily.

  • Domain

    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Alternative names

    • bB128O4.2.1 (interleukin 13 receptor, alpha 1)
    • Cancer/testis antigen 19
    • CD213a1
    • CD213A1 Antigen
    • CT 19
    • CT19
    • I13R1_HUMAN
    • IL 13 receptor subunit alpha 1
    • IL 13R alpha 1
    • IL 13R subunit alpha 1
    • IL 13Ra
    • IL 13RA1
    • IL-13 receptor subunit alpha-1
    • IL-13R subunit alpha-1
    • IL-13R-alpha-1
    • IL-13RA1
    • IL13 receptor alpha 1 chain
    • IL13R
    • IL13RA
    • Il13ra1
    • Interleukin 13 receptor alpha 1
    • Interleukin 13 receptor alpha 1 chain
    • interleukin 13 receptor subunit alpha 1
    • Interleukin-13 receptor subunit alpha-1
    • NR 4
    • NR4
    see all

  • Database links

    Images

    • Example data
      Example data

      This standard curve is for demonstration only. A standard curve must be run with each assay.

       

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