Anti-HELB antibody - C-terminal (ab202141)
Key features and details
- Rabbit polyclonal to HELB - C-terminal
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-HELB antibody - C-terminal -
Description
Rabbit polyclonal to HELB - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human HELB aa 1037-1087 (C terminal). The exact sequence is proprietary. NP_387467.2
Database link: Q8NG08 -
Positive control
- HeLa, 293T, Jurkat, TCMK-1 and NIH3T3 whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Images
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Western blot - Anti-HELB antibody - C-terminal (ab202141)All lanes : Anti-HELB antibody - C-terminal (ab202141) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 123 kDa
Exposure time: 30 seconds
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Immunoprecipitation - Anti-HELB antibody - C-terminal (ab202141)Lane 1: Detection of HELB in immunoprecipitates of 293T whole cell lysate (prepared using NETN lysis buffer; 0.5 or 1.0 mg per IP reaction, 20% of IP loaded) using ab202141 at 6 µg per reaction, and at 0.4 µg/ml for subsequent Western blot detection.
Lane 2: Control IgG
Detection: Chemiluminescence with an exposure time of 30 seconds.