Anti-GNAQ antibody (ab113429)
Key features and details
- Goat polyclonal to GNAQ
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-GNAQ antibody
See all GNAQ primary antibodies -
Description
Goat polyclonal to GNAQ -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Pig
Predicted to work with: Horse, Cow, Dog, Chinese hamster
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Immunogen
Synthetic peptide corresponding to Human GNAQ aa 150-250 (internal sequence) (Cysteine residue).
Database link: P50148 -
Positive control
- WB: Human, mouse and rat testis tissue lysates, peripheral blood lymphocytes lysate and pig colon and spleen tissue lysates. IHC-P: Human kidney tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab113429 was purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GNAQ antibody (ab113429)All lanes : Anti-GNAQ antibody (ab113429) at 0.1 µg/ml
Lane 1 : Mouse testis tissue lysate in RIPA buffer
Lane 2 : Rat testis tissue lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 42 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour. Detected using chemiluminescence.
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Western blot - Anti-GNAQ antibody (ab113429)Anti-GNAQ antibody (ab113429) at 0.1 µg/ml + Peripheral blood lymphocyte lysate in RIPA buffer at 35 µg
Predicted band size: 42 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour. Detected using chemiluminescence.
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Western blot - Anti-GNAQ antibody (ab113429)All lanes : Anti-GNAQ antibody (ab113429) at 0.1 µg/ml
Lane 1 : Pig colon tissue lysate in RIPA buffer
Lane 2 : Pig spleen tissue lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 42 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour. Detected using chemiluminescence.
