Anti-GNAQ antibody [EPR17149] (ab199533)
![Anti-GNAQ antibody [EPR17149] (ab199533)](https://www.abcam.com/ps/products/199/ab199533/Images/ab199533-242142-anti-gnaq-antibody-epr17149-western-blot.jpg "Anti-GNAQ antibody [EPR17149] (ab199533)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17149] to GNAQ
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-GNAQ antibody [EPR17149]
See all GNAQ primary antibodies -
Description
Rabbit monoclonal [EPR17149] to GNAQ -
Host species
Rabbit -
Specificity
The immunogen used for this product shares 77% homology with GNA11. Cross-reactivity with this protein has not been confirmed experimentally. -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HEK293, HeLa, HepG2, C6, PC-12 and NIH/3T3 whole cell lysates; Mouse brain, mouse kidney, mouse spleen, rat brain and rat kidney lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17149 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GNAQ antibody [EPR17149] (ab199533)All lanes : Anti-GNAQ antibody [EPR17149] (ab199533) at 1/1000 dilution
Lane 1 : HEK293 (Human embryonic kidney) whole cell lysate
Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 3 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-GNAQ antibody [EPR17149] (ab199533)](https://www.abcam.com/ps/products/199/ab199533/Images/ab199533-242141-anti-gnaq-antibody-epr17149-western-blot.jpg)
Western blot - Anti-GNAQ antibody [EPR17149] (ab199533)All lanes : Anti-GNAQ antibody [EPR17149] (ab199533) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Mouse spleen lysate
Lane 4 : Rat brain lysate
Lane 5 : Rat kidney lysate
Lane 6 : C6 (Rat glial tumor cells) whole cell lysate
Lane 7 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 8 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
-
![Anti-GNAQ antibody [EPR17149] (ab199533)](https://www.abcam.com/ps/products/199/ab199533/Images/ab199533-4-benefits-of-recombinant-antibodies.png)
Anti-GNAQ antibody [EPR17149] (ab199533)
