Recombinant Human FGFR1 alpha protein (Fc Chimera) (ab84000)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Suitable for: SDS-PAGE
-
Product name
Recombinant Human FGFR1 alpha protein (Fc Chimera)
See all FGFR1 alpha proteins and peptides -
Purity
> 95 % SDS-PAGE. -
Expression system
HEK 293 cells -
Accession
-
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Sequence
Theoretical Sequence: RPSPTLPEQAQPWGAPVEVESFLVHPGDLLQLRCRLRDD VQSINWLRDGVQLAESNRTRITGE EVEVQDSVPADSGLYACVTSSPSG SDTTYFSVNVSDALPSSEDDDDDDDSSSEEKETDNTKP NRMPVAPYWT SPEKMEKKLHAVPAAKTVKFKCPSSGTPNPTLRWLKNGKEFKPDHRIGGY KV RYATWSIIMDSVVPSDKGNYTCIVENEYGSINHTYQLDVVERSPHR PILQAGLPANKTVALGSN VEFMCKVYSDPQPHIQWLKHIEVNGSKIGP DNLPYVQILKTAGVNTTDKEMEVLHLRNVSFEDA GEYTCLAGNSIGLS HHSAWLTVLEALEGIPKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLF PPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKP REEQYNSTYRVVSVL TVLHQDWLNGKEYKCRVSNKALPAPIEKTISKA KGQPREPQVYTLPPSRDELTKNQVSLTCLVK GFYPSDIAVEWESNGQP ENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEAL HNH YTQKSLSLSPGK -
Amino acids
1 to 363 -
Additional sequence information
Encodes the signal peptide and extracellular domain of human FGF R1 alpha (IIIc) (aa 1-363) was fused to the Fc region IgG1 (aa 93-330). The chimeric protein was expressed in modified human 293 cells.
-
Preparation and Storage
- BFGFR
- CD331
- CEK
Images
-
Functional Studies - Recombinant Human FGFR1 alpha protein (Fc Chimera) (ab84000)Densitometry of protein isoforms visualised by 2-DE.
The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
The triangle indicates the theoretical MW and pI of the protein. -
SDS-PAGE - Recombinant Human FGFR1 alpha protein (Fc Chimera) (ab84000)1D SDS-PAGE of ab84000 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1: ab84000
Lane 2: ab84000 treated with PNGase F to remove potential N-linked glycans
Lane 3: ab84000 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.
10 μg protein was loaded per lane; Gel was stained with Deep Purple™. -
SDS-PAGE - Recombinant Human FGFR1 alpha protein (Fc Chimera) (ab84000)A sample of ab84000 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. 40 μg protein was loaded. Spot train (Deep Purple™ stained) indicates presence of multiple isoforms.
