Recombinant human Aurora A protein (Active) (ab271368)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 75% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human Aurora A protein (Active)
See all Aurora A proteins and peptides -
Biological activity
Specific Activity; 45 pmole/min/μg.Recombinant human Aurora A protein activity was 45 pmole/min/μg in a kinase assay using KEMPtide as substrate (0.2 mg/ml), containing 2 mM DTT using and 10 μM ATP in assay buffer. Reaction was done at 30°C for 45 min. Amount of ATP transferred was calculated using ADP-Glo reagent.
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Purity
>= 75 % SDS-PAGE. -
Expression system
HEK 293 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
DRSKENCISG PVKATAPVGG PKRVLVTQQF PCQNPLPVNS GQAQRVLCPS NSSQRVPLQA QKLVSSHKPV QNQKQKQLQA TSVPHPVSRP LNNTQKSKQP LPSAPENNPE EELASKQKNE ESKKRQWALE DFEIGRPLGK GKFGNVYLAR EKQSKFILAL KVLFKAQLEK AGVEHQLRRE VEIQSHLRHP NILRLYGYFH DATRVYLILE YAPLGTVYRE LQKLSKFDEQ RTATYITELA NALSYCHSKR VIHRDIKPEN LLLGSAGELK IADFGWSVHA PSSRRTTLCG TLDYLPPEMI EGRMHDEKVD LWSLGVLCYE FLVGKPPFEA NTYQETYKRI SRVEFTFPDF VTEGARDLIS RLLKHNPSQR PMLREVLEHP WITANSSKPS NCQNKESASK QS -
Predicted molecular weight
46 kDa including tags -
Amino acids
2 to 403 -
Tags
His tag N-Terminus
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Preservative: 1.36% Imidazole
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant human Aurora A protein (Active) (ab271368)
Recombinant human Aurora A protein activity was 45 pmole/min/μg in a kinase assay using KEMPtide as substrate (0.2 mg/ml).
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SDS-PAGE - Recombinant human Aurora A protein (Active) (ab271368)SDS-PAGE analysis of 2 µg ab271368.
