Antibodies, Proteins, Kits and Reagents for Life Science

Anti-GDF15 antibody [EPR19939] - BSA and Azide free (ab271973)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19939] to GDF15 - BSA and Azide free
Suitable for: ELISA, WB, IHC-P, IP
Reacts with: Human

Product name

Anti-GDF15 antibody [EPR19939] - BSA and Azide free
See all GDF15 primary antibodies
Description

Rabbit monoclonal [EPR19939] to GDF15 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: ELISA, WB, IHC-P, IPmore details
Species reactivity

Reacts with: Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HepG2 and LNCaP whole cell lysates; human prostate cancer and placenta lysates; untreated HT1080 and treated with 200ng/ml Phorbol-12-myristate-13-acetate (TPA) for 24 hours whole cell lysates.IHC-P: Human placenta and prostate hyperplasia tissues.IP: HepG2 whole cell lysate.
General notes
ab271973 is the carrier-free version of ab206414. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm.

Maxpar^® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19939
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF15 antibody [EPR19939] - BSA and Azide free (ab271973)

Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling GDF15 with ab206414 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasmic staining on human placenta is observed (PMID: 9593718). Positive staining on human placenta and prostate hyperplasia, other tissues tested were negative.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab206414).
Immunoprecipitation - Anti-GDF15 antibody [EPR19939] - BSA and Azide free (ab271973)

GDF15 was immunoprecipitated from 0.35 mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab206414 at 1/30 dilution.
Western blot was performed from the immunoprecipitate using ab206414 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: HepG2 whole cell lysate 10µg (Input).
Lane 2: ab206414 IP in HepG2 whole cell lysate.
Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab206414 in HepG2 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab206414).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF15 antibody [EPR19939] - BSA and Azide free (ab271973)

Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue labeling GDF15 with ab206414 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasmic staining on human prostate hyperplasia is observed (PMID: 9593718). Positive staining on human placenta and prostate hyperplasia, other tissues tested were negative.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab206414).
ELISA - Anti-GDF15 antibody [EPR19939] - BSA and Azide free (ab271973)

ELISA analysis of ab206414 binding functionally active mature GDF15 (ab50077) at 1 µg/mL.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab206414).
Anti-GDF15 antibody [EPR19939] - BSA and Azide free (ab271973)

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