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Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] - BSA and Azide free (ab188033)

Price and availability

526 012 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] - BSA and Azide free (ab188033)
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  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1375Y] to Liver Carboxylesterase 1/CES1 - BSA and Azide free
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] - BSA and Azide free
    See all Liver Carboxylesterase 1/CES1 primary antibodies
  • Description

    Rabbit monoclonal [EP1375Y] to Liver Carboxylesterase 1/CES1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow cyt: U-937 cells; WB: U-937 cells, mouse and rat kidney lysate.
  • General notes

    Ab188033 is the carrier-free version of ab68190. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab188033 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1375Y
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cholesterol Metabolism
    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Hydrolysis
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Signal Transduction
    • Metabolism
    • Drug metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Cholesterol Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Hydrolysis
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Drug metabolism

Images

  • Western blot - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] - BSA and Azide free (ab188033)
    Western blot - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] - BSA and Azide free (ab188033)
    All lanes : Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] (ab68190) at 1/50000 dilution (Purified)

    Lane 1 : U-937 (Human histiocytic lymphoma monocyte) whole cell lysate
    Lane 2 : Mouse kidney lysate
    Lane 3 : Rat kidney lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 62 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab68190, the same antibody clone in a different buffer formulation.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 21049984 and PMID: 30513753).

    Blocking Buffer and concentration: 5% NFDM/TBST

  • Flow Cytometry - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] - BSA and Azide free (ab188033)
    Flow Cytometry - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] - BSA and Azide free (ab188033)

    This data was developed using ab68190, the same antibody clone in a different buffer formulation.
    Flow Cytometry analysis of U-937 (Human histiocytic lymphoma monocyte) cells labeling Liver Carboxylesterase 1/CES1 with Purified ab188033 at 1:70 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488,ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] - BSA and Azide free (ab188033)
    Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] - BSA and Azide free (ab188033)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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