Anti-Liver Carboxylesterase 1/CES1 antibody (ab45957)
Key features and details
- Rabbit polyclonal to Liver Carboxylesterase 1/CES1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Liver Carboxylesterase 1/CES1 antibody
See all Liver Carboxylesterase 1/CES1 primary antibodies -
Description
Rabbit polyclonal to Liver Carboxylesterase 1/CES1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Baboon, Rhesus monkey
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Immunogen
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General notes
This product was previously labelled as Liver Carboxylesterase 1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab45957 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes IHC-P Use a concentration of 1 µg/ml. WB Use a concentration of 1 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa). ICC/IF Use a concentration of 1 µg/ml. Target
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Function
Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Catalyzes the transesterification of cocaine to form cocaethylene. Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate. -
Tissue specificity
Expressed predominantly in liver with lower levels in heart and lung. -
Sequence similarities
Belongs to the type-B carboxylesterase/lipase family. -
Post-translational
modificationsContains sialic acid.
Cleavage of the signal sequence can occur at 2 positions, either between Trp-17 and Gly-18 or between Gly-18 and His-19. -
Cellular localization
Endoplasmic reticulum lumen. - Information by UniProt
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Database links
- Entrez Gene: 1066 Human
- Entrez Gene: 12623 Mouse
- Entrez Gene: 24346 Rat
- Entrez Gene: 29225 Rat
- Omim: 114835 Human
- SwissProt: P23141 Human
- SwissProt: Q8VCC2 Mouse
- SwissProt: P10959 Rat
see all -
Alternative names
- ACAT antibody
- Acyl coenzyme A cholesterol acyltransferase antibody
- Acyl-coenzyme A:cholesterol acyltransferase antibody
see all
Images
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Western blot - Anti-Liver Carboxylesterase 1/CES1 antibody (ab45957)All lanes : Anti-Liver Carboxylesterase 1/CES1 antibody (ab45957) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole cell lysate
Lane 2 :THP-1 whole cell lysate (ab7913)
Lane 3 : Liver (Rat) Tissue Lysate - normal tissue
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 63 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Liver Carboxylesterase 1/CES1 antibody (ab45957)ICC/IF image of ab45957 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab45957, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive result in 100% Methanol fixed (5 min) HepG2 cells at 1µg/ml. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Carboxylesterase 1/CES1 antibody (ab45957)IHC image of Carboxylesterase 1 staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab45957, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
References (9)
ab45957 has been referenced in 9 publications.
- Xu Y et al. Hepatocyte-Specific Expression of Human Carboxylesterase 1 Attenuates Diet-Induced Steatohepatitis and Hyperlipidemia in Mice. Hepatol Commun 4:527-539 (2020). PubMed: 32258948
- Wen X et al. Hepatic carboxylesterases are differentially regulated in PPARa-null mice treated with perfluorooctanoic acid. Toxicology 416:15-22 (2019). PubMed: 30685356
- Xu Y et al. Lipocalin-2 Protects Against Diet-Induced Nonalcoholic Fatty Liver Disease by Targeting Hepatocytes. Hepatol Commun 3:763-775 (2019). PubMed: 31168511
- Xu J et al. Global inactivation of carboxylesterase 1 (Ces1/Ces1g) protects against atherosclerosis in Ldlr-/-mice. Sci Rep 7:17845 (2017). PubMed: 29259301
- Li J et al. Sortilin 1 Modulates Hepatic Cholesterol Lipotoxicity in Mice via Functional Interaction with Liver Carboxylesterase 1. J Biol Chem 292:146-160 (2017). PubMed: 27881673
- Fu J et al. Biotransformation Capacity of Carboxylesterase in Skin and Keratinocytes for the Penta-Ethyl Ester Prodrug of DTPA. Drug Metab Dispos 44:1313-8 (2016). PubMed: 27130352
- Xu J et al. Carboxylesterase 1 Is Regulated by Hepatocyte Nuclear Factor 4a and Protects Against Alcohol- and MCD diet-induced Liver Injury. Sci Rep 6:24277 (2016). WB . PubMed: 27075303
- Miwa S et al. Carboxylesterase converts Amplex red to resorufin: Implications for mitochondrial H2O2 release assays. Free Radic Biol Med 90:173-83 (2016). PubMed: 26577176
- Xu J et al. Hepatic carboxylesterase 1 is induced by glucose and regulates postprandial glucose levels. PLoS One 9:e109663 (2014). WB ; Mouse . PubMed: 25285996
Images
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Western blot - Anti-Liver Carboxylesterase 1/CES1 antibody (ab45957)All lanes : Anti-Liver Carboxylesterase 1/CES1 antibody (ab45957) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole cell lysate
Lane 2 :THP-1 whole cell lysate (ab7913)
Lane 3 : Liver (Rat) Tissue Lysate - normal tissue
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 63 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Liver Carboxylesterase 1/CES1 antibody (ab45957)ICC/IF image of ab45957 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab45957, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive result in 100% Methanol fixed (5 min) HepG2 cells at 1µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Carboxylesterase 1/CES1 antibody (ab45957)
IHC image of Carboxylesterase 1 staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab45957, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
