Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] (ab68190)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1375Y] to Liver Carboxylesterase 1/CES1
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y]
    See all Liver Carboxylesterase 1/CES1 primary antibodies

  • Description

    Rabbit monoclonal [EP1375Y] to Liver Carboxylesterase 1/CES1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Liver Carboxylesterase 1/CES1 (C terminal). The exact sequence is proprietary.

  • Positive control

    • Flow cyt: U-937 cells; WB: U-937 cells, mouse and rat kidney lysates.

  • General notes

     

     

     This product was previously labelled as Liver Carboxylesterase 1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] (ab68190)
    Western blot - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] (ab68190)
    All lanes : Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] (ab68190) at 1/50000 dilution (Purified)

    Lane 1 : U-937 (Human histiocytic lymphoma monocyte) whole cell lysate
    Lane 2 : Mouse kidney lysate
    Lane 3 : Rat kidney lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 62 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?



    The molecular weight observed is consistent with what has been described in the literature (PMID: 21049984 and PMID: 30513753).

    Blocking Buffer and concentration: 5% NFDM/TBST

  • Flow Cytometry - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] (ab68190)
    Flow Cytometry - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] (ab68190)
    Flow Cytometry analysis of U-937 (Human histiocytic lymphoma monocyte) cells labeling Liver Carboxylesterase 1/CES1 with Purified ab68190 at 1:70 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488,ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] (ab68190)
    Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] (ab68190)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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