Myristoylated Protein Assay Kit (Red) (ab273270)
Key features and details
- Assay type: Cell-based (qualitative)
- Detection method: Fluorescent
- Platform: Flow cytometer, Fluorescence microscope
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
Myristoylated Protein Assay Kit (Red)
See all Myristoylated Protein kits -
Detection method
Fluorescent -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based (qualitative) -
Assay duration
Multiple steps standard assay -
Product overview
Myristoylated Protein Activity Assay Kit (Red) (ab273270) is a highly specific, simple and robust tool for labeling and detection of myristoylated proteins. In this assay, an Alkynyl Myristic Acid is added directly to the cells and gets incorporated into proteins during or post translation followed by the click reaction with the azide-containing dye. This system offers a powerful method for imaging localization, trafficking and dynamics of Myristoylated proteins, or detection by FACS for quantitative studies. Labeled Myristoylated Proteins can be directly detected in 1D or 2D gels using the appropriate excitation sources, or enriched by immunoprecipitation with biotin-azide or antibodies prior to proteomic analysis. We provide sufficient materials for 100 assays in a 96-well plate format.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Tested applications
Suitable for: Flow Cyt, FMmore details -
Platform
Flow cytometer, Fluorescence microscope
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Copper Reagent (100X) 1 x 100µl Fixative Solution 1 x 10µl Fluorescent Azide (100X) 1 x 100µl Myristic Acid Label (1000X) 1 x 10µl Permeabilization Buffer (10X) 1 x 25ml Reducing Agent (20X) 1 x 500µl Total DNA Stain (1000X) 1 x 20µl Wash Buffer (X10) 1 x 25ml
Images
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Metabolic labeling of Myristic Acid in proliferating Jurkat cells.
Jurkat cells (1 x 106 cells) were incubated overnight with fresh aliquots of media containing Myristic Acid Label: Flow cytometry analysis of Negative Control (Black), Background (Fluorescent Azide only, Green)and Positive Control (Myristic Acid Label and Fluorescent Azide, Red). Signal measured in FL-2 channel clearly shows the Myristic Acid Labeling of Protein.
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Metabolic labeling of Myristic Acid in proliferating HeLa cells.HeLa cells (1 x 105 cells) incubated overnight with fresh aliquots of media containing Myristic Acid Label. Upper panel corresponds to the Fluorescent Azide only Background fluorescence on HeLa cells. The lower panel shows the red fluorescence corresponds to the Myristic Acid Label labeling reaction. Nuclear staining in both panels confirms that the red signal is a result of Myristic Acid Label incorporation.
