Antibodies, Proteins, Kits and Reagents for Life Science

247 259 ₸ Product size

100 µl 247 259 ₸

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Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (ab191887)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18869] to GABARAPL1 + GABARAPL3
Suitable for: WB, IP
Reacts with: Mouse, Rat, Human, Recombinant fragment

Product name

Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869]
See all GABARAPL1+GABARAPL3 primary antibodies
Description

Rabbit monoclonal [EPR18869] to GABARAPL1 + GABARAPL3
Host species

Rabbit

Tested Applications & Species

Application	Species
IP	Human
WB	Mouse Rat Human Recombinant fragment

See all applications and species data

Immunogen

Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human GABARAPL1 full length protein; Recombinant Human GABARAPL3 full length protein; Human fetal brain and fetal liver lysates; HeLa, HepG2, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Mouse and rat brain and kidney lysates. IP: HeLa whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18869
Isotype

IgG
Research areas

Western blot - Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (ab191887)

Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (ab191887) at 1/5000 dilution + Recombinant human GABARAPL1 full length protein at 0.01 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 14 kDa
Observed band size: 43 kDa
why is the actual band size different from the predicted?

Exposure time: 1 second

Blocking/Dilution buffer: 5% NFDM/TBST.
In house recombinant Human GABARAPL1 is a GST-tagged full length protein (aa1-117).
Western blot - Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (ab191887)

All lanes : Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (ab191887) at 1/5000 dilution

Lane 1 : Recombinant Human GABARAPL2 full length protein
Lane 2 : Recombinant Human GABARAPL3 full length protein
Lane 3 : Recombinant Human GABARAP (O95166) full length protein

Lysates/proteins at 0.01 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 14 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?

Exposure time: 1 second

Blocking/Dilution buffer: 5% NFDM/TBST.
All three recombinant Human full length proteins contain aa1-117 with an N-terminal His-Tag® and were made in-house.
Western blot - Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (ab191887)

All lanes : Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (ab191887) at 1/1000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal liver lysate
Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 14 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 15 seconds; Lane 2,3 and 4: 30 seconds.
The molecular weight observed is consistent with what has been described in the literature (PMID: 16650615).
Western blot - Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (ab191887)

All lanes : Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (ab191887) at 1/1000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Mouse kidney lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 14 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 5 seconds; Lane 2: 15 seconds.
The molecular weight observed is consistent with what has been described in the literature (PMID: 16650615).
Western blot - Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (ab191887)

All lanes : Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (ab191887) at 1/1000 dilution

Lane 1 : Rat brain lysate
Lane 2 : Rat kidney lysate
Lane 3 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 4 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 5 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 14 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?

Exposure time: 30 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 16650615).
Immunoprecipitation - Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (ab191887)

GABARAPL1 + GABARAPL3 was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab191887 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab191887 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: HeLa whole cell lysate 10ug (Input).

Lane 2: ab191887 IP in HeLa whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab191887 in HeLa whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 30 seconds.
Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (ab191887)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (ab191887)

Key features and details

Overview

Product name

Description

Host species

Tested Applications & Species

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Images

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