Anti-PCNA antibody (ab152112)
Key features and details
- Rabbit polyclonal to PCNA
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PCNA antibody
See all PCNA primary antibodies -
Description
Rabbit polyclonal to PCNA -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P MouseRatHumanIP HumanWB MouseHuman
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-253 of PCNA (Uniprot: P12004).
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Positive control
- Jurkat, Raji, 293T, A431, HeLa, HepG2, H1299, HCT116, MCF7, NT2D1, PC3, U87 MG, NIH 3T3, JC, BCL1, PC12, A549 and SiHa whole cell lysates; HeLa cells; Human KYSE170 xenograft and Human breast carcinoma tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PCNA antibody (ab152112)All lanes : Anti-PCNA antibody (ab152112) at 1/3000 dilution
Lane 1 : HCT116 whole cell lysate at 15 µg
Lane 2 : HCT116 whole cell lysate at 10 µg
Lane 3 : HCT116 whole cell lysate at 5 µg
Lane 4 : HCT116 whole cell lysate at 1 µg
Predicted band size: 29 kDa
12% SDS PAGE -
Immunoprecipitation - Anti-PCNA antibody (ab152112)PCBP2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extracts with 5 µg ab152112. Western blot was performed from the immunoprecipitate using ab152112.
Lane 1: PCPB2 protein only.
Lane 2: IgG instead of ab152112 in HeLa whole cell extract.
Lane 3: ab152112 IP in HeLa whole cell extract.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab152112)Paraffin-embedded mouseliver tissue stained for PCNA using ab152112 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab152112)Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PCNA with ab152112 at 1/500 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab152112)Immunohistochemical analysis of paraffin-embedded Human KYSE170 xenograft tissue labeling PCNA with ab152112 at 1/500 dilution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab152112)Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling PCNA with ab152112 at 1/500 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab152112)Immunohistochemical analysis of paraffin-embedded Rat hind brain tissue labeling PCNA with ab152112 at 1/500 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab152112)Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling PCNA with ab152112 at 1/500 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab152112)Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling PCNA with ab152112 at 1/100 dilution. -
Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody (ab152112)Immunofluorescent analysis of methanol-fixed HeLa cells labeling PCNA with ab152112 at 1/500 dilution. -
Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody (ab152112)Immunofluorescent analysis of HeLa (Human epithelial cells from cervix adenocarcinoma cell line) labeling PCNA with ab152112 at 1/500 dilution.
Counterstain is Hoechst 33342 (blue).
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Western blot - Anti-PCNA antibody (ab152112)All lanes : Anti-PCNA antibody (ab152112) at 1/3000 dilution
Lane 1 : Jurkat whole cell lysate
Lane 2 : Raji whole cell lysate
Lane 3 : 293T whole cell lysate
Lane 4 : A431 whole cell lysate
Lane 5 : HeLa whole cell lyate
Lane 6 : HepG2 whole cell lysate
Lane 7 : H1299 whole cell lysate
Lane 8 : HCT116 whole cell lysate
Lane 9 : MCF7 whole cell lysate
Lane 10 : NT2D1 whole cell lysate
Lane 11 : PC3 whole cell lysate
Lane 12 : U87 MG whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 29 kDa
12% SDS PAGE -
Western blot - Anti-PCNA antibody (ab152112)All lanes : Anti-PCNA antibody (ab152112) at 1/1000 dilution
Lane 1 : NIH 3T3 whole cell lysate
Lane 2 : JC whole cell lysate
Lane 3 : BCL1 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 29 kDa
12% SDS PAGE -
Western blot - Anti-PCNA antibody (ab152112)Anti-PCNA antibody (ab152112) at 1/1000 dilution + PC12 whole cell lysate at 30 µg
Predicted band size: 29 kDa
12% SDS PAGE -
Western blot - Anti-PCNA antibody (ab152112)All lanes : Anti-PCNA antibody (ab152112) at 1/3000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : NIH 3T3 whole cell lysate
Lane 3 : PC12 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 29 kDa
12% SDS PAGE -
Western blot - Anti-PCNA antibody (ab152112)Anti-PCNA antibody (ab152112) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg
Predicted band size: 29 kDa
12% SDS PAGE
