Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PCNA antibody [EPR3821] - BSA and Azide free (ab218310)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3821] to PCNA - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PCNA antibody [EPR3821] - BSA and Azide free
    See all PCNA primary antibodies

  • Description

    Rabbit monoclonal [EPR3821] to PCNA - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse spleen lysate. HeLa, NIH/3T3, PC-12, HepG2, HEK-293, HEK-293T and A431 cell lysates. IHC-P: Human ovarian carcinoma, urinary bladder carcinoma, normal colon, breast carcinoma and cervical carcinoma tissue. Rat liver tissue. Mouse testis tissue. ICC/IF: A431 and HeLa cells. IP: HeLa cell lysate. Flow Cyt: HeLa cells.

  • General notes

    Ab218310 is the carrier-free version of ab92552. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab218310 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [EPR3821] - BSA and Azide free (ab218310)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [EPR3821] - BSA and Azide free (ab218310)
    Immunohistochemical staining of paraffin embedded rat liver with purified ab92552 at a working dilution of 1/250. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92552).

  • Immunoprecipitation - Anti-PCNA antibody [EPR3821] - BSA and Azide free (ab218310)
    Immunoprecipitation - Anti-PCNA antibody [EPR3821] - BSA and Azide free (ab218310)

    ab92552 (purified) at 1/20 immunoprecipitating PCNA in 10 µg HeLa (Lanes 1 and 2, observed at 29 kDa). Lane 3 - PBS. For western blotting, HRP Veriblot for IP (ab131366) was used for detection at 1/10 000 dilution. Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92552).

  • Flow Cytometry - Anti-PCNA antibody [EPR3821] - BSA and Azide free (ab218310)
    Flow Cytometry - Anti-PCNA antibody [EPR3821] - BSA and Azide free (ab218310)
    Overlay histogram showing HeLa cells fixed in 4% PFA and stained with purified ab92552 at a dilution of 1 in 40 (red line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 500. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of both primary and secondary antibody were used as a negative control (blue line).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92552).

  • Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [EPR3821] - BSA and Azide free (ab218310)
    Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [EPR3821] - BSA and Azide free (ab218310)

    Immunofluorescence staining of A431 cells with purified ab92552 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab92552 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92552).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [EPR3821] - BSA and Azide free (ab218310)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [EPR3821] - BSA and Azide free (ab218310)
    Immunohistochemical staining of paraffin embedded mouse testis with purified ab92552 at a working dilution of 1/250. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92552).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [EPR3821] - BSA and Azide free (ab218310)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [EPR3821] - BSA and Azide free (ab218310)
    Immunohistochemical staining of paraffin embedded human cervical carcinoma with purified ab92552 at a working dilution of 1/250. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92552).

  • Flow Cytometry - Anti-PCNA antibody [EPR3821] - BSA and Azide free (ab218310)
    Flow Cytometry - Anti-PCNA antibody [EPR3821] - BSA and Azide free (ab218310)

    Overlay histogram showing HeLa cells stained with unpurified ab92552 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92552, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92552).

  • Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [EPR3821] - BSA and Azide free (ab218310)
    Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [EPR3821] - BSA and Azide free (ab218310) This image is courtesy of an Abreview submitted by Kirk McManus.

    Unpurified ab92552 (1/200) staining PCNA in Hela cells (green). Cells were fixed in methanol and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to abreview.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92552).

  • Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [EPR3821] - BSA and Azide free (ab218310)
    Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [EPR3821] - BSA and Azide free (ab218310)

    Unpurified ab92552 at 1/100 dilution staining PCNA in HeLa cells by Immunofluorescence.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92552).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [EPR3821] - BSA and Azide free (ab218310)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [EPR3821] - BSA and Azide free (ab218310)

    Unpurified ab92552 showing positive staining in human ovarian carcinoma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92552).

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [EPR3821] - BSA and Azide free (ab218310)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [EPR3821] - BSA and Azide free (ab218310)

    Unpurified ab92552 showing positive staining in human urinary bladder carcinoma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92552).

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [EPR3821] - BSA and Azide free (ab218310)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [EPR3821] - BSA and Azide free (ab218310)

    Unpurified ab92552 showing positive staining in human normal colon tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92552).

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [EPR3821] - BSA and Azide free (ab218310)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [EPR3821] - BSA and Azide free (ab218310)

    Unpurified ab92552 showing positive staining in human breast carcinoma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92552).

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

  • Anti-PCNA antibody [EPR3821] - BSA and Azide free (ab218310)
    Anti-PCNA antibody [EPR3821] - BSA and Azide free (ab218310)

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