Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ACBD3 antibody [EPR8623] (ab134952)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8623] to ACBD3
  • Suitable for: WB, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-ACBD3 antibody [EPR8623]
    See all ACBD3 primary antibodies

  • Description

    Rabbit monoclonal [EPR8623] to ACBD3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    A synthetic peptide corresponding to a region within Human ACBD3 (UniProt ID: Q9H3P7).

  • Positive control

    • K562, HeLa, 293T and LnCaP cell lysates. K562 cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ACBD3 antibody [EPR8623] (ab134952)
    Western blot - Anti-ACBD3 antibody [EPR8623] (ab134952)

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: ACBD3 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: HepG2 whole cell lysate (20 µg)

     Lanes 1 - 4: Merged signal (red and green). Green - ab134952 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.

     ab134952 was shown to specifically react with ACBD3 when ACBD3 knockout samples were used. Wild-type and ACBD3 knockout samples were subjected to SDS-PAGE. Ab134952 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-ACBD3 antibody [EPR8623] (ab134952)
    Western blot - Anti-ACBD3 antibody [EPR8623] (ab134952)
    All lanes : Anti-ACBD3 antibody [EPR8623] (ab134952) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : LNCaP cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti rabbit at 1/2000 dilution

    Predicted band size: 61 kDa

  • Flow Cytometry - Anti-ACBD3 antibody [EPR8623] (ab134952)
    Flow Cytometry - Anti-ACBD3 antibody [EPR8623] (ab134952)
    Flow cytometric analysis of permeabilized K562 cells labelling ACBD3 using ab134952 (red) at 1/100 dilution and rabbit IgG negative control (green).
  • OI-RD Scanning - Anti-ACBD3 antibody [EPR8623] (ab134952)
    OI-RD Scanning - Anti-ACBD3 antibody [EPR8623] (ab134952)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-ACBD3 antibody [EPR8623] (ab134952)
    Anti-ACBD3 antibody [EPR8623] (ab134952)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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