Anti-HOOK2 antibody (ab89696)
Key features and details
- Rabbit polyclonal to HOOK2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-HOOK2 antibody
See all HOOK2 primary antibodies -
Description
Rabbit polyclonal to HOOK2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Synthetic peptide corresponding to Mouse HOOK2 aa 700 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab101733) -
Positive control
- This antibody gave a positive signal in human brain tissue lysate.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentrationConcentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab89696 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).Target
-
Relevance
HOOK2 is a member of the Hook protein family. Hook proteins are cytosolic coiled-coil proteins that contain conserved N-terminal domains, which attach to microtubules, and more divergent C-terminal domains, which mediate binding to organelles. HOOK2 localizes to the centrosome through all phases of the cell cycle. Interference with HOOK2 function results in the loss of the radial organization of microtubules suggesting that HOOK2 contributes to the establishment and maintenance of centrosomal structure and function. HOOK2 may contribute to the establishment and maintenance of the pericentrosomal localization of aggresomes by promoting the microtubule-based delivery of protein aggregates to pericentriolar aggresomes. -
Cellular localization
Cytoplasmic. Note=Associates with discrete punctate structure that correspond to neither early or late endosomes, lysosomes, multivesicular bodies (MVBs), Golgi complex, endoplasmic reticulum, nor mitochondria. -
Database links
- Entrez Gene: 29911 Human
- Entrez Gene: 170833 Mouse
- Entrez Gene: 304669 Rat
- Omim: 607824 Human
- SwissProt: Q96ED9 Human
- SwissProt: Q7TMK6 Mouse
- Unigene: 30792 Human
- Unigene: 247775 Mouse
see all -
Alternative names
- A630054I03Rik antibody
- FLJ26218 antibody
- h hook2 antibody
see all
Images
-
Anti-HOOK2 antibody (ab89696) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 83 kDa
Additional bands at: 23 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Datasheets and documents
References (0)
ab89696 has not yet been referenced specifically in any publications.
Images
-
Anti-HOOK2 antibody (ab89696) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 83 kDa
Additional bands at: 23 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes