Anti-PCNA antibody - Nuclear Loading Control (ab139696)
Key features and details
- Chicken polyclonal to PCNA - Nuclear Loading Control
- Suitable for: WB
- Reacts with: Human
- Isotype: IgY
Overview
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Product name
Anti-PCNA antibody - Nuclear Loading Control
See all PCNA primary antibodies -
Description
Chicken polyclonal to PCNA - Nuclear Loading Control -
Host species
Chicken -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Macaque monkey, Chinese hamster
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Immunogen
Synthetic peptide within Human PCNA aa 200-300 (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
(Peptide available asab139977)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 3% BSA
This product may contain up to 3% BSA depending on the batch. For specific batch formulations please contact us. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Images
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Western blot - Anti-PCNA antibody - Nuclear Loading Control (ab139696)All lanes : Anti-PCNA antibody - Nuclear Loading Control (ab139696) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 20 µg
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252) at 20 µg
Lane 4 :Recombinant Human PCNA protein (ab85651) at 0.2 µg
Secondary
All lanes : Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: 4 minutes
