Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TDO2/TDO antibody [EPR23745-311] - BSA and Azide free (ab281571)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23745-311] to TDO2/TDO - BSA and Azide free
  • Suitable for: WB, IP, ICC
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TDO2/TDO antibody [EPR23745-311] - BSA and Azide free
    See all TDO2/TDO primary antibodies

  • Description

    Rabbit monoclonal [EPR23745-311] to TDO2/TDO - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, ICCmore details
    Unsuitable for: Flow Cyt (Intra),IHC-Fr or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human liver, Mouse liver, Rat liver lysates. ICC: 293T cells. IP: Human liver, Mouse liver lysates.

  • General notes

    ab281571 is the carrier-free version of ab259359. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab281571 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Constituent: 100% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23745-311

  • Isotype

    IgG

Images

  • Western blot - Anti-TDO2 antibody [EPR23745-311] - BSA and Azide free (ab281571)
    Western blot - Anti-TDO2 antibody [EPR23745-311] - BSA and Azide free (ab281571)
    All lanes : Anti-TDO2/TDO antibody [EPR23745-311] (ab259359) at 1/1000 dilution

    Lane 1 : Human liver tissue lysate
    Lane 2 : Human pancreas tissue lysate at 40 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution


    This data was developed using 259359, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The the expression pattern and observed MW is consistent with what has been described in the literature (PMID: 27573671, PMID: 25691885)

    Negative control: pancreas (PMID:25691885).

     Exposure time: 26 seconds.

     

  • Western blot - Anti-TDO2 antibody [EPR23745-311] - BSA and Azide free (ab281571)
    Western blot - Anti-TDO2 antibody [EPR23745-311] - BSA and Azide free (ab281571)
    All lanes : Anti-TDO2/TDO antibody [EPR23745-311] (ab259359) at 1/1000 dilution

    Lane 1 : Mouse liver tissue lysate
    Lane 2 : Mouse pancreas tissue lysate
    Lane 3 : Rat liver tissue lysate
    Lane 4 : Rat pancreas tissue lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Observed band size: 43 kDa why is the actual band size different from the predicted?



    This data was developed using 259359, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression pattern and observed MW is consistent with what has been described in the literature (PMID:25691885, PMID: 10775460).

    Negative control: pancreas (PMID:25691885).

     Exposure time: 26 seconds.

     

  • Immunocytochemistry - Anti-TDO2 antibody [EPR23745-311] - BSA and Azide free (ab281571)
    Immunocytochemistry - Anti-TDO2 antibody [EPR23745-311] - BSA and Azide free (ab281571)

    This data was developed using ab259359, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T cells labelling TDO2/TDO with ab259359 at 1/500 (1.072 ug/ml) dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) antibody at 1/500 dilution (Green) Confocal image showing cytoplasmic staining in 293T cells transfected with myc-tagged TDO2 expression vector. is observed. Myc-Tag Mouse mAb (Alexa Fluor® 488) was used to counterstain tubulin at 1/100 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/500 dilution.

  • Immunoprecipitation - Anti-TDO2 antibody [EPR23745-311] - BSA and Azide free (ab281571)
    Immunoprecipitation - Anti-TDO2 antibody [EPR23745-311] - BSA and Azide free (ab281571)

    This data was developed using ab259359, the same antibody clone in a different buffer formulation.

    TDO2/TDO was immunoprecipitated from 0.35 mg Human liver tissue lysate 10 ug with ab259359 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259359 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Human liver tissue lysate 10 ug

    Lane 2: ab259359 IP in Human liver tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab259359 in human liver tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

     

  • Immunoprecipitation - Anti-TDO2 antibody [EPR23745-311] - BSA and Azide free (ab281571)
    Immunoprecipitation - Anti-TDO2 antibody [EPR23745-311] - BSA and Azide free (ab281571)

    This data was developed using ab259359, the same antibody clone in a different buffer formulation.

    TDO2/TDO was immunoprecipitated from 0.35 mg Mouse liver tissue lysate 10 ug with ab259359 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259359 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse liver tissue lysate 10 ug

    Lane 2: ab259359 IP in Mouse liver tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab259359 in mouse liver tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

     

  • Anti-TDO2 antibody [EPR23745-311] - BSA and Azide free (ab281571)
    Anti-TDO2 antibody [EPR23745-311] - BSA and Azide free (ab281571)

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