Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SFT antibody [EPR13000(B)] (ab176561)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13000(B)] to SFT
  • Suitable for: WB, IHC-P, Flow Cyt, IP
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-SFT antibody [EPR13000(B)]
    See all SFT primary antibodies

  • Description

    Rabbit monoclonal [EPR13000(B)] to SFT

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    within Human SFT aa 100 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P51668

  • Positive control

    • Jurkat and Raji cell lysates, Human skeletal muscle lysate, Human thryoid gland carcinoma and prostate tissues, Jurkat cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SFT antibody [EPR13000(B)] (ab176561)
    Western blot - Anti-SFT antibody [EPR13000(B)] (ab176561)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: SFT knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: Jurkat cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab176561 observed at 18 kDa. Red - loading control, ab181602, observed at 37 kDa. ab176561 was shown to recognize SFT when SFT knockout samples were used, along with additional cross-reactive bands. Wild-type and SFT knockout samples were subjected to SDS-PAGE. ab176561 and ab181602 (loading control to GAPDH) were diluted 1 μg/mL and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-SFT antibody [EPR13000(B)] (ab176561)
    Western blot - Anti-SFT antibody [EPR13000(B)] (ab176561)
    All lanes : Anti-SFT antibody [EPR13000(B)] (ab176561) at 1/10000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : Raji cell lysate
    Lane 3 : Human skeletal muscle lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 17 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFT antibody [EPR13000(B)] (ab176561)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFT antibody [EPR13000(B)] (ab176561)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human prostate tissue labeling SFT with ab176561 at a 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFT antibody [EPR13000(B)] (ab176561)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFT antibody [EPR13000(B)] (ab176561)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human thyroid gland carcinoma tissue labeling SFT with ab176561 at a 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-SFT antibody [EPR13000(B)] (ab176561)
    Flow Cytometry - Anti-SFT antibody [EPR13000(B)] (ab176561)

    Flow cytometry analysis of permeabilized Jurkat cells labeling SFT (red) with ab176561 at a 1/10 dilution, or negative control rabbit IgG (green)

  • Immunoprecipitation - Anti-SFT antibody [EPR13000(B)] (ab176561)
    Immunoprecipitation - Anti-SFT antibody [EPR13000(B)] (ab176561)

    Western blot analysis on immunoprecipitation pellet from Human skeletal muscle lysate labeling SFT with ab176561 at 1/10 dilution.

  • Anti-SFT antibody [EPR13000(B)] (ab176561)
    Anti-SFT antibody [EPR13000(B)] (ab176561)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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