Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cytochrome C antibody [EPR1327] (ab133504)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1327] to Cytochrome C
  • Suitable for: WB, IHC-P, ICC/IF, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Cytochrome C antibody [EPR1327]
    See all Cytochrome C primary antibodies

  • Description

    Rabbit monoclonal [EPR1327] to Cytochrome C

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Mouse
    Rat
    Human
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Cytochrome C aa 1-100. The exact sequence is proprietary.
    Database link: P99999

  • Positive control

    • WB: Human fetal kidney and fetal heart tissue lysate. Rat and mouse brain tissue lysate. Molt4 and SH-SY5Y cell lysate. Human heart, kidney and spleen lysate. ICC/IF: SH-SY5Y and HeLa cells. IHC-P: Human cervical carcinoma tissue. Mouse liver tissue. Human and rat kidney tissue. IP: Molt-4 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Cytochrome C antibody [EPR1327] (ab133504)
    Western blot - Anti-Cytochrome C antibody [EPR1327] (ab133504)
    Anti-Cytochrome C antibody [EPR1327] (ab133504) at 1/5000 dilution (purified) + Human fetal kidney tissue lysate at 10 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 11 kDa
    Observed band size: 14 kDa
    why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [EPR1327] (ab133504)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [EPR1327] (ab133504)

    Immunofluorescence staining of SH-SY5Y cells with purified ab133504 at a working dilution of 1 in 100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti rabbit (ab150077), used at a dilution of 1 in 500. ab7291 was used to stain tubulin, and this is shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom middle and right hand panels - for the negative controls, purified ab133504 was used at a dilution of 1/200 followed by an Alexa Fluor® 594 goat anti-mouse antibody at a dilution of 1/500.

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [EPR1327] (ab133504)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [EPR1327] (ab133504)

    Immunofluorescent analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labelling Cytochrome C with unpurified ab133504 at 1/100 dilution.

  • Western blot - Anti-Cytochrome C antibody [EPR1327] (ab133504)
    Western blot - Anti-Cytochrome C antibody [EPR1327] (ab133504)
    Anti-Cytochrome C antibody [EPR1327] (ab133504) at 1/5000 dilution (purified) + Human fetal heart tissue lysate at 10 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 11 kDa
    Observed band size: 14 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-Cytochrome C antibody [EPR1327] (ab133504)
    Western blot - Anti-Cytochrome C antibody [EPR1327] (ab133504)
    Anti-Cytochrome C antibody [EPR1327] (ab133504) at 1/5000 dilution (purified) + Rat brain tissue lysate at 1/1000 dilution

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 11 kDa
    Observed band size: 14 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-Cytochrome C antibody [EPR1327] (ab133504)
    Western blot - Anti-Cytochrome C antibody [EPR1327] (ab133504)
    Anti-Cytochrome C antibody [EPR1327] (ab133504) at 1/50000 dilution (purified) + Mouse brain tissue lysate at 10 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 11 kDa
    Observed band size: 14 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EPR1327] (ab133504)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EPR1327] (ab133504)

    Immunohistochemical staining of paraffin embedded human cervical carcinoma with purified ab133504 at a working dilution of 1 in 500. The secondary antibody used is a HRP goat anti-rabbit H+L (ab97051). The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EPR1327] (ab133504)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EPR1327] (ab133504)

    Immunohistochemical staining of paraffin embedded mouse liver with purified ab133504 at a working dilution of 1 in 500. The secondary antibody used is a HRP goat anti-rabbit H+L (ab97051). The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EPR1327] (ab133504)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EPR1327] (ab133504)

    Immunohistochemical staining of paraffin embedded rat kidney with purified ab133504 at a working dilution of 1 in 500. The secondary antibody used is a HRP goat anti-rabbit H+L (ab97051). The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Immunoprecipitation - Anti-Cytochrome C antibody [EPR1327] (ab133504)
    Immunoprecipitation - Anti-Cytochrome C antibody [EPR1327] (ab133504)

    ab133504 (purified) at 1/30 immunoprecipitating Cytochrome C in Molt-4 cells.

    For western blotting, a HRP-conjugated goat anti-rabbit (H+L), was used as the secondary antibody (1/1000).

    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

  • Western blot - Anti-Cytochrome C antibody [EPR1327] (ab133504)
    Western blot - Anti-Cytochrome C antibody [EPR1327] (ab133504)
    All lanes : Anti-Cytochrome C antibody [EPR1327] (ab133504) at 1/10000 dilution (unpurified)

    Lane 1 : Molt4 lysate
    Lane 2 : SH-SY5Y lysate
    Lane 3 : Human heart lysate
    Lane 4 : Human kidney lysate
    Lane 5 : Human spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 11 kDa
    Observed band size: 14 kDa why is the actual band size different from the predicted?

  • OI-RD Scanning - Anti-Cytochrome C antibody [EPR1327] (ab133504)
    OI-RD Scanning - Anti-Cytochrome C antibody [EPR1327] (ab133504)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EPR1327] (ab133504)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EPR1327] (ab133504)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labelling Cytochrome C with unpurified ab133504 at 1/250 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-Cytochrome C antibody [EPR1327] (ab133504)
    Anti-Cytochrome C antibody [EPR1327] (ab133504)

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