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Anti-ATG7 antibody (ab223380)

Anti-ATG7 antibody (ab223380)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to ATG7
  • Suitable for: ICC/IF, WB
  • Reacts with: Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-ATG7 antibody
    See all ATG7 primary antibodies
  • Description

    Rabbit polyclonal to ATG7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human ATG7 (N terminal).
    Database link: O95352

  • Positive control

    • WB: Rat brain cell lysate; HeLa whole cell lysate. ICC/IF: RKO cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Associated Proteins
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ub-like Proteins
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Cardiovascular
    • Heart
    • Autophagy
    • APG gene products
    • Cancer
    • Signal transduction
    • Autophagy
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitophagy fission and fusion
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Autophagy and mitophagy
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Autophagy and mitophagy
    • APG gene products
    • Cancer
    • Cell Death
    • Autophagy
    • Signal Transduction
    • Cancer
    • Cell Death
    • Autophagy
    • APG gene products

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-ATG7 antibody (ab223380)
    Immunocytochemistry/ Immunofluorescence - Anti-ATG7 antibody (ab223380)

    4% formaldehyde-fixed RKO cells stained for ATG7 (green) using ab223380 at 1/100 dilution in ICC/IF. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1/100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 min at RT, 5 min at RT. 

    (A) DAPI nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223380 (D) Merge.

  • Western blot - Anti-ATG7 antibody (ab223380)
    Western blot - Anti-ATG7 antibody (ab223380)
    Anti-ATG7 antibody (ab223380) at 1/1000 dilution + Rat brain cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG HRP at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 69,75,78 kDa
    Observed band size: 78 kDa
    why is the actual band size different from the predicted?


    Exposure time: 6 minutes


    Blocking buffer: 2% BSA and 2% skim milk in TBST.

    Primary antibody was incubated for 16 hours at 4°C, the secondary for 1 hour at RT.

  • Western blot - Anti-ATG7 antibody (ab223380)
    Western blot - Anti-ATG7 antibody (ab223380)
    Anti-ATG7 antibody (ab223380) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG HRP at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 69,75,78 kDa
    Observed band size: 78 kDa why is the actual band size different from the predicted?


    Exposure time: 6 minutes


    Blocking buffer: 5% skim milk in TBST.

    Primary and secondary antibodies were incubated for 1 hour at RT.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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