Anti-ATG7 antibody (ab223380)
Key features and details
- Rabbit polyclonal to ATG7
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-ATG7 antibody
See all ATG7 primary antibodies -
Description
Rabbit polyclonal to ATG7 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide corresponding to Human ATG7 (N terminal).
Database link: O95352 -
Positive control
- WB: Rat brain cell lysate; HeLa whole cell lysate. ICC/IF: RKO cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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4% formaldehyde-fixed RKO cells stained for ATG7 (green) using ab223380 at 1/100 dilution in ICC/IF. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1/100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 min at RT, 5 min at RT.
(A) DAPI nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223380 (D) Merge.
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Anti-ATG7 antibody (ab223380) at 1/1000 dilution + Rat brain cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 69,75,78 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?
Exposure time: 6 minutesBlocking buffer: 2% BSA and 2% skim milk in TBST.
Primary antibody was incubated for 16 hours at 4°C, the secondary for 1 hour at RT.
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Anti-ATG7 antibody (ab223380) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 69,75,78 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?
Exposure time: 6 minutesBlocking buffer: 5% skim milk in TBST.
Primary and secondary antibodies were incubated for 1 hour at RT.