Human UMOD Matched Antibody Pair Kit (ab221421)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 62.5 pg/ml - 8000 pg/ml
Overview
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Product name
Human UMOD Matched Antibody Pair Kit
See all UMOD kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
46.9 pg/ml -
Range
62.5 pg/ml - 8000 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Human UMOD Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human UMOD.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Human UMOD Capture Antibody 2 x 50µg 1 x 50µg Human UMOD Detector Antibody 2 x 12.5µg 1 x 12.5µg Human UMOD Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Not known. May play a role in regulating the circulating activity of cytokines as it binds to IL-1, IL-2 and TNF with high affinity. -
Tissue specificity
Synthesized by kidney. Most abundant protein in normal human urine. -
Involvement in disease
Defects in UMOD are the cause of familial juvenile hyperuricemic nephropathy type 1 (HNFJ1) [MIM:162000]. HNFJ1 is a renal disease characterized by juvenil onset of hyperuricemia, polyuria, progressive renal failure, and gout. The disease is associated with interstitial pathological changes resulting in fibrosis.
Defects in UMOD are the cause of medullary cystic kidney disease type 2 (MCKD2) [MIM:603860]. MCKD2 is a form of tubulointerstitial nephropathy characterized by formation of renal cysts at the corticomedullary junction. It is characterized by adult onset of impaired renal function and salt wasting resulting in end-stage renal failure by the sixth decade.
Defects in UMOD are the cause of glomerulocystic kidney disease with hyperuricemia and isosthenuria (GCKDHI) [MIM:609886]. GCKDHI is a renal disorder characterized by a cystic dilation of Bowman space, a collapse of glomerular tuft, and hyperuricemia due to low fractional excretion of uric acid and severe impairment of urine concentrating ability. -
Sequence similarities
Contains 3 EGF-like domains.
Contains 1 ZP domain. -
Cellular localization
Cell membrane. Secreted. Secreted after cleavage in the urine. - Information by UniProt
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Alternative names
- ADMCKD2
- FJHN
- HNFJ
see all -
Database links
- Entrez Gene: 7369 Human
- Omim: 191845 Human
- SwissProt: P07911 Human
- Unigene: 654425 Human
Images
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Human Uromodulin standard curve
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Human UMOD Matched Antibody Pair Kit (ab221421)To learn more about the advantages of recombinant antibodies see here.
