Anti-CD105 antibody [OTI8A1] (ab156756)
![Anti-CD105 antibody [OTI8A1] (ab156756)](https://www.abcam.com/ps/products/156/ab156756/Images/ab156756-201943-anti-cd105-antibody-oti8a1-flow-cytometry.jpg "Anti-CD105 antibody [OTI8A1] (ab156756)")
Key features and details
- Mouse monoclonal [OTI8A1] to CD105
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Mouse, Rat, Dog, Human, African green monkey
- Isotype: IgG2b
Overview
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Product name
Anti-CD105 antibody [OTI8A1]
See all CD105 primary antibodies -
Description
Mouse monoclonal [OTI8A1] to CD105 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanWB Human
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Immunogen
Recombinant full length protein corresponding to Human CD105.
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Positive control
- WB: HepG2, HeLa, SVT2, A549, COS7, Jurkat, MDCK, PC12, and MCF7 cell lysates. IF: HeLa cells.
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General notes
The clone number has been updated from 8A1 to OTI8A1, both clone numbers name the same clone.
This product was changed from ascites to tissue culture supernatant on 29th May 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 1% BSA, 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab156756 is purified from TCS by affinity chromatography. -
Clonality
Monoclonal -
Clone number
OTI8A1 -
Isotype
IgG2b -
Research areas
Images
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Flow Cytometry - Anti-CD105 antibody [OTI8A1] (ab156756)Overlay histogram showing U937 cells stained with ab156756 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab156756, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in U937 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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![Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [OTI8A1] (ab156756)](https://www.abcam.com/ps/products/156/ab156756/Images/ab156756-163817-anti-cd105-antibody-oti8a1-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [OTI8A1] (ab156756)Immunofluorescent analysis of HeLa cells labeling CHN1 with ab156756 at 1/100. -
![Western blot - Anti-CD105 antibody [OTI8A1] (ab156756)](https://www.abcam.com/ps/products/156/ab156756/Images/ab156756-163816-anti-cd105-antibody-oti8a1-western-blot.jpg)
Western blot - Anti-CD105 antibody [OTI8A1] (ab156756)All lanes : Anti-CD105 antibody [OTI8A1] (ab156756) at 1/200 dilution
Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control
Lane 2 : HEK293T cells transfected with pCMV6-ENTRY CD105
Lysates/proteins at 5 µg per lane.
Predicted band size: 70 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC211854) -
![Western blot - Anti-CD105 antibody [OTI8A1] (ab156756)](https://www.abcam.com/ps/products/156/ab156756/Images/ab156756-163815-anti-cd105-antibody-oti8a1-western-blot.jpg)
Western blot - Anti-CD105 antibody [OTI8A1] (ab156756)All lanes : Anti-CD105 antibody [OTI8A1] (ab156756) at 1/200 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : SVT2 cell lysate
Lane 4 : A549 cell lysate
Lane 5 : COS7 cell lysate
Lane 6 : Jurkat cell lysate
Lane 7 : MDCK cell lysate
Lane 8 : PC12 cell lysate
Lane 9 : MCF7 cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 70 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC211696)
